B1L8W7 (FMT_THESQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	TRQ2_0409

Organism

Thermotoga sp. (strain RQ2) [Complete proteome] [HAMAP]

Taxonomic identifier

126740 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Thermotogae › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182
Sequence similarities	Belongs to the fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182		PRO_1000098454
Regions
	Region	109 – 112	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1L8W7 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 48055C0CDEF4FD92	FASTA	313	35,216
	10 20 30 40 50 60 MRIVFVGTPE FAAEILEHLI KNGFNVVGVV TQPDKPRGRG RKVEPTPVKV VAEKHRVPFI 70 80 90 100 110 120 QPESINKKEA LEFLRSVGPD VIIVASYGKI LGEKVLSLPS LGCYNIHPSL LPKYRGASPI 130 140 150 160 170 180 QRVLENGEER TGVTIYKMVR ELDAGPIALQ REISIDPFET FDQLEKRLIE LSKEMSIEFL 190 200 210 220 230 240 EKLKVGDIEL KEQDHSRATY APMIKKEDLI VDFSKDAESV KNKIRAYDSR PGARAFLGND 250 260 270 280 290 300 EVKLFGVTAI DSSGDEPGLI HYIDREGAWI GTGKGMVKVK YLQLPGKKKL TFWELRNGRL 310 IEEGMKLEGR YES

References

[1]

"Complete sequence of Thermotoga sp. RQ2." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Bruce D.B., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RQ2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000969 Genomic DNA. Translation: ACB08765.1.
RefSeq	YP_001738448.1. NC_010483.1.
3D structure databases
ProteinModelPortal	B1L8W7.
ModBase	Search...
Protein-protein interaction databases
STRING	126740.TRQ2_0409.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB08765; ACB08765; TRQ2_0409.
GeneID	6091814.
KEGG	trq:TRQ2_0409.
PATRIC	23947331. VBITheSp108950_0414.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
OMA	GITLMQM.
ProtClustDB	CLSK875200.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_THESQ

Accession

Primary (citable) accession number: B1L8W7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program