B1L8G0 (B1L8G0_THESQ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193
EC=6.3.1.5 HAMAP MF_00193

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	TRQ2_0283

Organism

Thermotoga sp. (strain RQ2) [Complete proteome] [HAMAP] EMBL ACB08642.1

Taxonomic identifier

126740 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 SAAS SAAS003694
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 SAAS SAAS003694 NAD HAMAP MF_00193 SAAS SAAS003694 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 SAAS SAAS003694
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	24 – 31	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	26	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	B1L8G0 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 77847379037EDF9E	FASTA	281	32,410
	10 20 30 40 50 60 MKESLINFIK EKIEEYNYRG AVVGVSGGID SAVVLSLCVQ ALGKDRVFGL ILPERDSSKD 70 80 90 100 110 120 SVKDAVELCE SLKVEYKIKP ITPILRKIGV YRLFPPKLFF PESVVKRYVL NKWNSLSKDP 130 140 150 160 170 180 FLDDLRNSGP NEFLKGLAYY RIKHRIRMCL LYFEAEKRGY AVVGTTNRTE YLTGLYVKWG 190 200 210 220 230 240 DEAVDIEPIM HLYKTQVFEL ARELNIPEKI QKKPPSPDLI PGVTDEMAFG MSYMELDRIL 250 260 270 280 MKIDRGESLE DEDPEKVERV KKILELAEKH RRGIPISFDR I

References

[1]

"Complete sequence of Thermotoga sp. RQ2." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Bruce D.B., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RQ2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000969 Genomic DNA. Translation: ACB08642.1.
RefSeq	YP_001738325.1. NC_010483.1.
3D structure databases
ProteinModelPortal	B1L8G0.
ModBase	Search...
Protein-protein interaction databases
STRING	B1L8G0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6091687.
GenomeReviews	Gene locus TRQ2_0283 in contig CP000969_GR.
KEGG	trq:TRQ2_0283.
PATRIC	23947078. VBITheSp108950_0289.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	DQTDADE.
ProtClustDB	CLSK875266.
Enzyme and pathway databases
BioCyc	TSP28240:TRQ2_0283-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01916.
Pfam	PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 2 hits.
ProtoNet	Search...

Entry information

Entry name

B1L8G0_THESQ

Accession

Primary (citable) accession number: B1L8G0

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	December 14, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)