Reviewed, UniProtKB/Swiss-Prot B1L7K1 (G1PDH_KORCO)

Names and origin

Protein names

Recommended name:
    Glycerol-1-phosphate dehydrogenase [NAD(P)+]
      Short name=G1P dehydrogenase
      Short name=G1PDH
    EC=1.1.1.261
Alternative name(s):
    sn-glycerol-1-phosphate dehydrogenase
    Enantiomeric glycerophosphate synthase

Gene names

Name:	egsA
Ordered Locus Names:	Kcr_0068

Organism

Korarchaeum cryptofilum (strain OPF8) [Complete proteome] [HAMAP]

Taxonomic identifier

374847 [NCBI]

Taxonomic lineage

Archaea › Korarchaeota › Candidatus Korarchaeum

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea By similarity.
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP MF_00497
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-dehydroquinate synthase activity Inferred from electronic annotation. Source: InterPro glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497		PRO_0000350647
Regions
	Nucleotide binding	52 – 56	5	NAD By similarity
	Nucleotide binding	74 – 77	4	NAD By similarity
Sites
	Metal binding	131	1	Zinc; catalytic By similarity
	Metal binding	211	1	Zinc; catalytic By similarity
	Metal binding	231	1	Zinc; catalytic By similarity
	Binding site	79	1	Substrate By similarity
	Binding site	83	1	NAD By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	215	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1L7K1-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 2CF8136B5A00503F	FASTA	314	33,618
	10 20 30 40 50 60 MTRAVAEDLA SSLEPDYRIL HVLTTGGLED LMDAIKTARK SEVDLLIGVG GGKPLDITKI 70 80 90 100 110 120 LAAELGVRYV VVPTSASHDG IASPSVSFTL SREIEERFGR VIRVEAPTAI LADTTIINRA 130 140 150 160 170 180 SPITFKSGFG DLVAKITAVR DWELAYKLRD EPYSEYAASM SLLSAKIAMD HAHEIRTRLE 190 200 210 220 230 240 ESTRILVKAL IGSGVAMSIA GSSRPASGSE HMFSHALDIL SSEAGVKPAP HGIQVAIGTI 250 260 270 280 290 300 MMAYLQGQDW KMIKEKLIEA GVPTTAEEAG ISPDMIVKAL TIAHKIRERY TILGSSGLTL 310 SAAEKLARVT GVIK

References

[1]

"A korarchaeal genome reveals new insights into the evolution of the Archaea." Elkins J.G., Podar M., Graham D.E., Makarova K.S., Wolf Y., Randau L., Hedlund B.P., Brochier-Armanet C., Kunin V., Anderson I., Lapidus A., Goltsman E., Barry K., Koonin E.V., Hugenholtz P., Kyrpides N., Wanner G., Richardson P., Keller M., Stetter K.O. Proc. Natl. Acad. Sci. U.S.A. 105:8102-8107(2008) [PubMed: 18535141] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000968 Genomic DNA. Translation: ACB06828.1.
RefSeq	YP_001736511.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6093357.
GenomeReviews	Gene locus Kcr_0068 in contig CP000968_GR.
KEGG	kcr:Kcr_0068.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ADWELAH.
Family and domain databases
HAMAP	MF_00497. [Tree]
InterPro	IPR002658. DHQ_synth_AroB. [Graphical view]
Pfam	PF01761. DHQ_synthase. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G1PDH_KORCO

Accession

Primary (citable) accession number: B1L7K1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	April 29, 2008
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)