B1L744 (B1L744_KORCO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit HAMAP MF_00324
Short name=Pol II HAMAP MF_00324
EC=2.7.7.7 HAMAP MF_00324

Gene names

Name:	polC HAMAP MF_00324
Ordered Locus Names:	Kcr_1527

Organism

Korarchaeum cryptofilum (strain OPF8)

Taxonomic identifier

374847 [NCBI]

Taxonomic lineage

Archaea › Korarchaeota › Candidatus Korarchaeum

Protein attributes

Sequence length	1115 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324 Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity. HAMAP MF_00324
Sequence similarities	Belongs to the archaeal DNA polymerase II family. HAMAP MF_00324

Ontologies

Keywords
Biological process	DNA replication HAMAP MF_00324
Ligand	DNA-binding HAMAP MF_00324
Molecular function	DNA-directed DNA polymerase HAMAP MF_00324 Exonuclease HAMAP MF_00324 Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme HAMAP MF_00324 Reference proteome
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B1L744 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: FE58260C6713D613	FASTA	1,115	125,754
	10 20 30 40 50 60 MDYFDTLRKG MDELLSVARR ARSLGLDPSN DIEISLANEL HERIAALFGI PELGERVKYW 70 80 90 100 110 120 LDATGSKLET AFRVIGEIVP GYYLKISYER RAELALRVGM AIITDATVSA PIEGISKVEV 130 140 150 160 170 180 KKQGGTYLSV YYNGPIRTAG GTEGAISVLM ADYIRQRLGI DRYRPTQEEI ERYVEEVSLY 190 200 210 220 230 240 KRIAHLQYNS EPNEVRIAVS NLPVEITGPP TEKEEVSSFR NLPRVETNRV RGGAVLVIND 250 260 270 280 290 300 CIIQKAKKLK KIIDQIKKIG FDDSCWGWIE GKKEEESEEE AGGCPVIEPS YKYLKDAVMG 310 320 330 340 350 360 RPVLSHPSRI GGFRLRYGRA RNTGLASIGM NPATMYLLDS FIAVGTQVRI ERPGKSAVVM 370 380 390 400 410 420 PVDSIEGPTV RLRDGSVIRV DDPDEAMRIM DQVEEIIHLG DVLIGYGEFL ENNHPLLPSA 430 440 450 460 470 480 WVEEWWEALV RRKGLRAVRG FEDALRASRE LDVPLHPRFT YYWEHITSEE LLKLAGAIRN 490 500 510 520 530 540 SERAEIDLNE DIKRILELLG VPHRVQNGKI VLDEEDWKAL SYIASKLPES LELPRYATIK 550 560 570 580 590 600 VLNQYLDVKV MPKGPTKIGM RVGRPEKAKP RKMKPPVNLL FPVGNLKGSS RSLDLAYEKG 610 620 630 640 650 660 SVNVEVALRL CRKCGSRTTL FRCPNCGSPT YKISFCPICG KQVNGKCQDH PNAEPVPYRS 670 680 690 700 710 720 VKLNIREEVD RALRKLGESP GILERVKGVK GLTSGSKIPE PIEKGILRAK HGLSVFKDGT 730 740 750 760 770 780 VRFDAVNAVL THFKPEEIGV SVEKLRELGY LEDVEGKYLE REDQLLELKV QDIIIPRAAA 790 800 810 820 830 840 KYLLSIANYI DELLVKFYGL EPFYNLRDES DLIGQLVLTI SPHTFVANLA RIIGFTNADV 850 860 870 880 890 900 IFAHPFLHAA KRRNVDGDED SIMLALDALI NFSREFLPSS PGGREDAPLL LVTKVDPKYI 910 920 930 940 950 960 DDEVYNMEVS ELPPEFYEAT YKFEDPSKLK GIIETVGSRI EAGDLYPKIV GSIETSRMDL 970 980 990 1000 1010 1020 GPLQTTYRKL ETMSDKMEAH FSLERKIRAV DEKDALSRAL TSHFLRDIIG NLRKFGQQEF 1030 1040 1050 1060 1070 1080 RCSECNAKYR RPPISGRCPR CGGNIVLTVH KGTVLKYLKP TLELIRRYGM EGYLSQRVKL 1090 1100 1110 IEGEISSLFK EEKINSADLS RFLCNERKVK LVDFL

References

[1]

"A korarchaeal genome reveals new insights into the evolution of the Archaea." Elkins J.G., Podar M., Graham D.E., Makarova K.S., Wolf Y., Randau L., Hedlund B.P., Brochier-Armanet C., Kunin V., Anderson I., Lapidus A., Goltsman E., Barry K., Koonin E.V., Hugenholtz P., Kyrpides N., Wanner G., Richardson P., Keller M., Stetter K.O. Proc. Natl. Acad. Sci. U.S.A. 105:8102-8107(2008) [PubMed: 18535141] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OPF8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000968 Genomic DNA. Translation: ACB08273.1.
RefSeq	YP_001737956.1. NC_010482.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B1L744.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6094804.
GenomeReviews	Gene locus Kcr_1527 in contig CP000968_GR.
KEGG	kcr:Kcr_1527.
Phylogenomic databases
HOGENOM	HBG497074.
OMA	NCDGDED.
PhylomeDB	B1L744.
ProtClustDB	PRK04023.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
KO	K02322.
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. PolC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B1L744_KORCO

Accession

Primary (citable) accession number: B1L744

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	December 14, 2011
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)