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B1L719

- PNPH_KORCO

UniProt

B1L719 - PNPH_KORCO

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Protein

Probable 6-oxopurine nucleoside phosphorylase

Gene

Kcr_1502

Organism
Korarchaeum cryptofilum (strain OPF8)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage.UniRule annotation

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101PhosphateUniRule annotation
Sitei173 – 1731Important for substrate specificityUniRule annotation
Binding sitei191 – 1911Substrate; via amide nitrogenUniRule annotation
Binding sitei192 – 1921PhosphateUniRule annotation
Sitei228 – 2281Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. purine-nucleoside phosphorylase activity Source: UniProtKB-EC
  3. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro

GO - Biological processi

  1. purine ribonucleoside salvage Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciKCRY374847:GH12-1540-MONOMER.
UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 6-oxopurine nucleoside phosphorylaseUniRule annotation (EC:2.4.2.1UniRule annotation)
Alternative name(s):
Purine nucleoside phosphorylaseUniRule annotation
Short name:
PNPUniRule annotation
Gene namesi
Ordered Locus Names:Kcr_1502
OrganismiKorarchaeum cryptofilum (strain OPF8)
Taxonomic identifieri374847 [NCBI]
Taxonomic lineageiArchaeaKorarchaeotaCandidatus Korarchaeum
ProteomesiUP000001686: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270Probable 6-oxopurine nucleoside phosphorylasePRO_0000415085Add
BLAST

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi374847.Kcr_1502.

Structurei

3D structure databases

ProteinModelPortaliB1L719.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 492Phosphate bindingUniRule annotation
Regioni215 – 2173Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
InParanoidiB1L719.
KOiK00772.
OMAiHVVGMTA.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.

Sequencei

Sequence statusi: Complete.

B1L719 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVRVGIIGGS GLYELLENPR IVRLDTPFGH CDVQLGELAG EEVAFIPRHG
60 70 80 90 100
ASHRLPPYKV NYKANLYALN MLEVERIIAT NAVGSINPAL EPGTIVIPHD
110 120 130 140 150
FIDMTKCRDV TFYDGETTIK VRGREVSGVV HVSMTPYTYC PEIRASIIEA
160 170 180 190 200
AEHMGLGVRD GGVYVCTEGN RFETPAEIRA FSILGGDIVG MTGCPEASLA
210 220 230 240 250
RELAICYASI SVVTNYAAGV SGAVKLTQGE VIEIFSRKIQ DISRLIEETI
260 270
RRIPKRRECP CKDALSEYLK
Length:270
Mass (Da):29,588
Last modified:April 29, 2008 - v1
Checksum:i69C874638AF82A82
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000968 Genomic DNA. Translation: ACB08248.1.
RefSeqiYP_001737931.1. NC_010482.1.

Genome annotation databases

EnsemblBacteriaiACB08248; ACB08248; Kcr_1502.
GeneIDi6094779.
KEGGikcr:Kcr_1502.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000968 Genomic DNA. Translation: ACB08248.1 .
RefSeqi YP_001737931.1. NC_010482.1.

3D structure databases

ProteinModelPortali B1L719.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 374847.Kcr_1502.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB08248 ; ACB08248 ; Kcr_1502 .
GeneIDi 6094779.
KEGGi kcr:Kcr_1502.

Phylogenomic databases

eggNOGi COG0005.
HOGENOMi HOG000228987.
InParanoidi B1L719.
KOi K00772.
OMAi HVVGMTA.

Enzyme and pathway databases

UniPathwayi UPA00606 .
BioCyci KCRY374847:GH12-1540-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1580. 1 hit.
HAMAPi MF_01963. MTAP.
InterProi IPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view ]
PANTHERi PTHR11904. PTHR11904. 1 hit.
Pfami PF01048. PNP_UDP_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53167. SSF53167. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OPF8.

Entry informationi

Entry nameiPNPH_KORCO
AccessioniPrimary (citable) accession number: B1L719
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: April 29, 2008
Last modified: October 29, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3