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B1L719

- PNPH_KORCO

UniProt

B1L719 - PNPH_KORCO

Protein

Probable 6-oxopurine nucleoside phosphorylase

Gene

Kcr_1502

Organism
Korarchaeum cryptofilum (strain OPF8)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage.UniRule annotation

    Catalytic activityi

    Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101PhosphateUniRule annotation
    Sitei173 – 1731Important for substrate specificityUniRule annotation
    Binding sitei191 – 1911Substrate; via amide nitrogenUniRule annotation
    Binding sitei192 – 1921PhosphateUniRule annotation
    Sitei228 – 2281Important for substrate specificityUniRule annotation

    GO - Molecular functioni

    1. phosphorylase activity Source: InterPro
    2. purine-nucleoside phosphorylase activity Source: UniProtKB-EC
    3. S-methyl-5-thioadenosine phosphorylase activity Source: InterPro

    GO - Biological processi

    1. purine ribonucleoside salvage Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    BioCyciKCRY374847:GH12-1540-MONOMER.
    UniPathwayiUPA00606.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable 6-oxopurine nucleoside phosphorylaseUniRule annotation (EC:2.4.2.1UniRule annotation)
    Alternative name(s):
    Purine nucleoside phosphorylaseUniRule annotation
    Short name:
    PNPUniRule annotation
    Gene namesi
    Ordered Locus Names:Kcr_1502
    OrganismiKorarchaeum cryptofilum (strain OPF8)
    Taxonomic identifieri374847 [NCBI]
    Taxonomic lineageiArchaeaKorarchaeotaCandidatus Korarchaeum
    ProteomesiUP000001686: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 270270Probable 6-oxopurine nucleoside phosphorylasePRO_0000415085Add
    BLAST

    Interactioni

    Subunit structurei

    Homohexamer. Dimer of a homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi374847.Kcr_1502.

    Structurei

    3D structure databases

    ProteinModelPortaliB1L719.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni48 – 492Phosphate bindingUniRule annotation
    Regioni215 – 2173Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0005.
    HOGENOMiHOG000228987.
    KOiK00772.
    OMAiHVVGMTA.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B1L719-1 [UniParc]FASTAAdd to Basket

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    MVRVGIIGGS GLYELLENPR IVRLDTPFGH CDVQLGELAG EEVAFIPRHG    50
    ASHRLPPYKV NYKANLYALN MLEVERIIAT NAVGSINPAL EPGTIVIPHD 100
    FIDMTKCRDV TFYDGETTIK VRGREVSGVV HVSMTPYTYC PEIRASIIEA 150
    AEHMGLGVRD GGVYVCTEGN RFETPAEIRA FSILGGDIVG MTGCPEASLA 200
    RELAICYASI SVVTNYAAGV SGAVKLTQGE VIEIFSRKIQ DISRLIEETI 250
    RRIPKRRECP CKDALSEYLK 270
    Length:270
    Mass (Da):29,588
    Last modified:April 29, 2008 - v1
    Checksum:i69C874638AF82A82
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000968 Genomic DNA. Translation: ACB08248.1.
    RefSeqiYP_001737931.1. NC_010482.1.

    Genome annotation databases

    EnsemblBacteriaiACB08248; ACB08248; Kcr_1502.
    GeneIDi6094779.
    KEGGikcr:Kcr_1502.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000968 Genomic DNA. Translation: ACB08248.1 .
    RefSeqi YP_001737931.1. NC_010482.1.

    3D structure databases

    ProteinModelPortali B1L719.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 374847.Kcr_1502.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB08248 ; ACB08248 ; Kcr_1502 .
    GeneIDi 6094779.
    KEGGi kcr:Kcr_1502.

    Phylogenomic databases

    eggNOGi COG0005.
    HOGENOMi HOG000228987.
    KOi K00772.
    OMAi HVVGMTA.

    Enzyme and pathway databases

    UniPathwayi UPA00606 .
    BioCyci KCRY374847:GH12-1540-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    HAMAPi MF_01963. MTAP.
    InterProi IPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    [Graphical view ]
    PANTHERi PTHR11904. PTHR11904. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53167. SSF53167. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: OPF8.

    Entry informationi

    Entry nameiPNPH_KORCO
    AccessioniPrimary (citable) accession number: B1L719
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 37 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3