Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B1L719 (PNPH_KORCO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable 6-oxopurine nucleoside phosphorylase

EC=2.4.2.1
Alternative name(s):
Purine nucleoside phosphorylase
Short name=PNP
Gene names
Ordered Locus Names:Kcr_1502
OrganismKorarchaeum cryptofilum (strain OPF8) [Reference proteome] [HAMAP]
Taxonomic identifier374847 [NCBI]
Taxonomic lineageArchaeaKorarchaeotaCandidatus Korarchaeum

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage By similarity. HAMAP-Rule MF_01963

Catalytic activity

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate. HAMAP-Rule MF_01963

Pathway

Purine metabolism; purine nucleoside salvage. HAMAP-Rule MF_01963

Subunit structure

Homohexamer. Dimer of a homotrimer By similarity. HAMAP-Rule MF_01963

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines By similarity.

Sequence similarities

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 270270Probable 6-oxopurine nucleoside phosphorylase HAMAP-Rule MF_01963
PRO_0000415085

Regions

Region48 – 492Phosphate binding By similarity
Region215 – 2173Substrate binding By similarity

Sites

Binding site101Phosphate By similarity
Binding site1911Substrate; via amide nitrogen By similarity
Binding site1921Phosphate By similarity
Site1731Important for substrate specificity By similarity
Site2281Important for substrate specificity By similarity

Sequences

Sequence LengthMass (Da)Tools
B1L719 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 69C874638AF82A82

FASTA27029,588
        10         20         30         40         50         60 
MVRVGIIGGS GLYELLENPR IVRLDTPFGH CDVQLGELAG EEVAFIPRHG ASHRLPPYKV 

        70         80         90        100        110        120 
NYKANLYALN MLEVERIIAT NAVGSINPAL EPGTIVIPHD FIDMTKCRDV TFYDGETTIK 

       130        140        150        160        170        180 
VRGREVSGVV HVSMTPYTYC PEIRASIIEA AEHMGLGVRD GGVYVCTEGN RFETPAEIRA 

       190        200        210        220        230        240 
FSILGGDIVG MTGCPEASLA RELAICYASI SVVTNYAAGV SGAVKLTQGE VIEIFSRKIQ 

       250        260        270 
DISRLIEETI RRIPKRRECP CKDALSEYLK 

« Hide

References

[1]"A korarchaeal genome reveals new insights into the evolution of the Archaea."
Elkins J.G., Podar M., Graham D.E., Makarova K.S., Wolf Y., Randau L., Hedlund B.P., Brochier-Armanet C., Kunin V., Anderson I., Lapidus A., Goltsman E., Barry K., Koonin E.V., Hugenholtz P., Kyrpides N., Wanner G., Richardson P., Keller M., Stetter K.O.
Proc. Natl. Acad. Sci. U.S.A. 105:8102-8107(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OPF8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000968 Genomic DNA. Translation: ACB08248.1.
RefSeqYP_001737931.1. NC_010482.1.

3D structure databases

ProteinModelPortalB1L719.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING374847.Kcr_1502.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB08248; ACB08248; Kcr_1502.
GeneID6094779.
KEGGkcr:Kcr_1502.

Phylogenomic databases

eggNOGCOG0005.
HOGENOMHOG000228987.
KOK00772.
OMAHVVGMTA.

Enzyme and pathway databases

BioCycKCRY374847:GH12-1540-MONOMER.
UniPathwayUPA00606.

Family and domain databases

Gene3D3.40.50.1580. 1 hit.
HAMAPMF_01963. MTAP.
InterProIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERPTHR11904. PTHR11904. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMSSF53167. SSF53167. 1 hit.
ProtoNetSearch...

Entry information

Entry namePNPH_KORCO
AccessionPrimary (citable) accession number: B1L719
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: April 29, 2008
Last modified: May 14, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways