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B1L6E9 (RTCA_KORCO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA 3'-terminal phosphate cyclase

Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4
Gene names
Name:rtcA
Ordered Locus Names:Kcr_1282
OrganismKorarchaeum cryptofilum (strain OPF8) [Reference proteome] [HAMAP]
Taxonomic identifier374847 [NCBI]
Taxonomic lineageArchaeaKorarchaeotaCandidatus Korarchaeum

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP-Rule MF_00200

Catalytic activity

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP-Rule MF_00200

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00200.

Sequence similarities

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-3'-phosphate cyclase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348RNA 3'-terminal phosphate cyclase HAMAP-Rule MF_00200
PRO_1000099349

Regions

Nucleotide binding285 – 2884ATP By similarity

Sites

Active site3111Tele-AMP-histidine intermediate By similarity
Binding site1021ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1L6E9 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: BFCEEB4468665F36

FASTA34837,666
        10         20         30         40         50         60 
MEFIRIDGSY GEGGGSLLRY AIALSSVTMK PVEIYNIRVK RANPGLRPQH LNAVRALARI 

        70         80         90        100        110        120 
TEATVEGDEV GSTALRFIPR KRAGGSFEID IGTAGSISLI IQAILPACIS SEEEISLRIR 

       130        140        150        160        170        180 
GGTDVPLAPP IDYMAEVFLR NMAPLGVRAE LKLLRRGHYP RGGGIVELHA SPSKLFPIDK 

       190        200        210        220        230        240 
VRGEKFDRVL GRCHAVKLPR SVVERISSSA IDTLRKEGLR VEIEEEWSED GHLGPGAGIV 

       250        260        270        280        290        300 
LWTDSNPRIG ADELGEKGKP SEVVGKNAAS KLLDEIKAGM AFDSHMGDMI IPYLALARGR 

       310        320        330        340 
SRVGISKLTL HAESNIWLVE RFLPVKFIVQ GGVGSPTVIE VEGAGLEL 

« Hide

References

[1]"A korarchaeal genome reveals new insights into the evolution of the Archaea."
Elkins J.G., Podar M., Graham D.E., Makarova K.S., Wolf Y., Randau L., Hedlund B.P., Brochier-Armanet C., Kunin V., Anderson I., Lapidus A., Goltsman E., Barry K., Koonin E.V., Hugenholtz P., Kyrpides N., Wanner G., Richardson P., Keller M., Stetter K.O.
Proc. Natl. Acad. Sci. U.S.A. 105:8102-8107(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OPF8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000968 Genomic DNA. Translation: ACB08028.1.
RefSeqYP_001737711.1. NC_010482.1.

3D structure databases

ProteinModelPortalB1L6E9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING374847.Kcr_1282.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB08028; ACB08028; Kcr_1282.
GeneID6094559.
KEGGkcr:Kcr_1282.

Phylogenomic databases

eggNOGCOG0430.
HOGENOMHOG000015264.
KOK01974.
OMARRGHYPK.

Enzyme and pathway databases

BioCycKCRY374847:GH12-1292-MONOMER.

Family and domain databases

Gene3D3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPMF_00200. RTC.
InterProIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERPTHR11096. PTHR11096. 1 hit.
PfamPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRTCA_KORCO
AccessionPrimary (citable) accession number: B1L6E9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: February 19, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families