B1L6E9 (RTCA_KORCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase
Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	Kcr_1282

Organism

Korarchaeum cryptofilum (strain OPF8)

Taxonomic identifier

374847 [NCBI]

Taxonomic lineage

Archaea › Korarchaeota › Candidatus Korarchaeum

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_1000099349
Regions
	Nucleotide binding	285 – 288	4	ATP By similarity
Sites
	Active site	311	1	Tele-AMP-histidine intermediate By similarity
	Binding site	102	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1L6E9 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: BFCEEB4468665F36	FASTA	348	37,666
	10 20 30 40 50 60 MEFIRIDGSY GEGGGSLLRY AIALSSVTMK PVEIYNIRVK RANPGLRPQH LNAVRALARI 70 80 90 100 110 120 TEATVEGDEV GSTALRFIPR KRAGGSFEID IGTAGSISLI IQAILPACIS SEEEISLRIR 130 140 150 160 170 180 GGTDVPLAPP IDYMAEVFLR NMAPLGVRAE LKLLRRGHYP RGGGIVELHA SPSKLFPIDK 190 200 210 220 230 240 VRGEKFDRVL GRCHAVKLPR SVVERISSSA IDTLRKEGLR VEIEEEWSED GHLGPGAGIV 250 260 270 280 290 300 LWTDSNPRIG ADELGEKGKP SEVVGKNAAS KLLDEIKAGM AFDSHMGDMI IPYLALARGR 310 320 330 340 SRVGISKLTL HAESNIWLVE RFLPVKFIVQ GGVGSPTVIE VEGAGLEL

References

[1]

"A korarchaeal genome reveals new insights into the evolution of the Archaea." Elkins J.G., Podar M., Graham D.E., Makarova K.S., Wolf Y., Randau L., Hedlund B.P., Brochier-Armanet C., Kunin V., Anderson I., Lapidus A., Goltsman E., Barry K., Koonin E.V., Hugenholtz P., Kyrpides N., Wanner G., Richardson P., Keller M., Stetter K.O. Proc. Natl. Acad. Sci. U.S.A. 105:8102-8107(2008) [PubMed: 18535141] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OPF8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000968 Genomic DNA. Translation: ACB08028.1.
RefSeq	YP_001737711.1. NC_010482.1.
3D structure databases
ProteinModelPortal	B1L6E9.
ModBase	Search...
Protein-protein interaction databases
STRING	B1L6E9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6094559.
GenomeReviews	Gene locus Kcr_1282 in contig CP000968_GR.
KEGG	kcr:Kcr_1282.
Phylogenomic databases
HOGENOM	HBG683539.
OMA	GGTDVAW.
PhylomeDB	B1L6E9.
Enzyme and pathway databases
BioCyc	CKOR374847:KCR_1282-MONOMER.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.30.360.20. G3DSA:3.30.360.20. 1 hit. G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 2 hits.
KO	K01974.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 2 hits.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_KORCO

Accession

Primary (citable) accession number: B1L6E9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program