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Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Korarchaeum cryptofilum (strain OPF8)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K(+)UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei242 – 2421NADUniRule annotation
Metal bindingi294 – 2941Potassium; via carbonyl oxygenUniRule annotation
Metal bindingi296 – 2961Potassium; via carbonyl oxygenUniRule annotation
Binding sitei297 – 2971IMPUniRule annotation
Active sitei299 – 2991Thioimidate intermediateUniRule annotation
Metal bindingi299 – 2991Potassium; via carbonyl oxygenUniRule annotation
Binding sitei408 – 4081IMPUniRule annotation
Metal bindingi462 – 4621Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi292 – 2943NADUniRule annotation

GO - Molecular functioni

  1. IMP dehydrogenase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-HAMAP
  3. nucleotide binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. GMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Potassium

Enzyme and pathway databases

BioCyciKCRY374847:GH12-1082-MONOMER.
UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenaseUniRule annotation
Short name:
IMPDUniRule annotation
Short name:
IMPDHUniRule annotation
Gene namesi
Name:guaBUniRule annotation
Ordered Locus Names:Kcr_1078
OrganismiKorarchaeum cryptofilum (strain OPF8)
Taxonomic identifieri374847 [NCBI]
Taxonomic lineageiArchaeaKorarchaeotaCandidatus Korarchaeum
ProteomesiUP000001686 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476Inosine-5'-monophosphate dehydrogenasePRO_0000415693Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi374847.Kcr_1078.

Structurei

3D structure databases

ProteinModelPortaliB1L5U5.
SMRiB1L5U5. Positions 9-92.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini93 – 15159CBS 1UniRule annotationAdd
BLAST
Domaini152 – 21160CBS 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni334 – 3363IMP bindingUniRule annotation
Regioni357 – 3582IMP bindingUniRule annotation
Regioni381 – 3855IMP bindingUniRule annotation

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation
Contains 2 CBS domains.UniRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiCOG0516.
HOGENOMiHOG000165755.
InParanoidiB1L5U5.
KOiK00088.
OMAiSSMGYCG.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1L5U5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIDRFEFAF TFNDVILLPG KTEIEPSNVD LTTRIGNIAL SIPILSSPMD
60 70 80 90 100
TVTEEEMSIA MARMGGLGIL HRNCSVEEQV NMAKAVKRAE SFIIRDVITV
110 120 130 140 150
SPEDSVEEAR RLMREHGISG LPVIVGRKLV GIVTRRDVYF AENGSLLVKD
160 170 180 190 200
IMTKDPITVG PEITPQEARK IMARYKIEKL PVVSESGELI GLVTAKDVFY
210 220 230 240 250
RESHPFATRD EEGRLRVGAA ISPFDIDRAK TLAPYVDVLV TDVAHFHNEN
260 270 280 290 300
VISATKRIID EVGVPVIAGN IGTYEAAEEA ITRLDIIGLR VGIGSGSICT
310 320 330 340 350
TGEVTGVAAP TLYAVASASE AVRKYSKDVA VIADGGIRGP GEAAKAFAMG
360 370 380 390 400
ADAVMLGYAL AGTKEAPGST MMIGGKMYKI YRGMGSPSAR SKRFAMDRYS
410 420 430 440 450
KPSKDIAEGI EGLVPYRGDV TTVVDRFVAG LKAAFGYVGA ANISEMKSKA
460 470
RVALISHSGM SEIAPHDVKP LEKISD
Length:476
Mass (Da):51,195
Last modified:April 28, 2008 - v1
Checksum:i6AAC31F930E62260
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000968 Genomic DNA. Translation: ACB07824.1.
RefSeqiYP_001737507.1. NC_010482.1.

Genome annotation databases

EnsemblBacteriaiACB07824; ACB07824; Kcr_1078.
GeneIDi6094355.
KEGGikcr:Kcr_1078.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000968 Genomic DNA. Translation: ACB07824.1.
RefSeqiYP_001737507.1. NC_010482.1.

3D structure databases

ProteinModelPortaliB1L5U5.
SMRiB1L5U5. Positions 9-92.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi374847.Kcr_1078.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB07824; ACB07824; Kcr_1078.
GeneIDi6094355.
KEGGikcr:Kcr_1078.

Phylogenomic databases

eggNOGiCOG0516.
HOGENOMiHOG000165755.
InParanoidiB1L5U5.
KOiK00088.
OMAiSSMGYCG.

Enzyme and pathway databases

UniPathwayiUPA00601; UER00295.
BioCyciKCRY374847:GH12-1082-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OPF8.

Entry informationi

Entry nameiIMDH_KORCO
AccessioniPrimary (citable) accession number: B1L5U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2012
Last sequence update: April 28, 2008
Last modified: March 3, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.