B1L3K8 (DNLI1_KORCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase 1
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] 1

Gene names

Name:	lig1
Ordered Locus Names:	Kcr_0279

Organism

Korarchaeum cryptofilum (strain OPF8)

Taxonomic identifier

374847 [NCBI]

Taxonomic lineage

Archaea › Korarchaeota › Candidatus Korarchaeum

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity. HAMAP MF_00407
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 541	541	DNA ligase 1 HAMAP MF_00407		PRO_0000365249
Sites
	Active site	236	1	N6-AMP-lysine intermediate By similarity
	Binding site	234	1	ATP By similarity
	Binding site	241	1	ATP By similarity
	Binding site	256	1	ATP By similarity
	Binding site	286	1	ATP By similarity
	Binding site	325	1	ATP By similarity
	Binding site	398	1	ATP By similarity
	Binding site	404	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1L3K8 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: D74B685AF3E7AC31	FASTA	541	61,819
	10 20 30 40 50 60 MLLKELAELA MRIASTSSRR DKVSLVSDLI RRSDPEEAYK ALLILTGRIF PPSDPRELNV 70 80 90 100 110 120 SWATLWKVVS SLSGAEPTGV DAGELVKSIV ERRGKRQTAL LEEPLTVEEV YKILEAISEA 130 140 150 160 170 180 EGPGSKGKRE ALLTIPFSRA DPSEAWLLAN AIVGETRLGL NEGLLIESIA QAYGLRKEDV 190 200 210 220 230 240 ERAVMVLGDP YEIVRRGGKL EFEPVLFRPL KPMLAQSSDS LRSAIEELGR CALEYKLDGV 250 260 270 280 290 300 RVQVHKRGDE VRFFSRRMSD ITKSLPDVSD QVRYGVRAGE AILEGELIAE RDGRPLPFQI 310 320 330 340 350 360 LIRRFKRRQL DPRLIEEIPL KLYIFDLILL DGSSYLRKPY FERREKLEGL IEDPVSLVRS 370 380 390 400 410 420 LITSDPDEGM DFMEEALREG HEGVIAKKLN SPYIPGVRGR YWLKVKETNS LDLVIVAAER 430 440 450 460 470 480 GYGRRHRWYS DYYLAARDPE SGEFLIVGKT FKGLTDEEFE WITQKLEELS IGKEGKLIRV 490 500 510 520 530 540 RPRIVVEVSF NEIQRSPKYK SGFALRFARI TRIRDDKSPE EADTIERVRE IYEKQIRKFQ L

References

[1]

"A korarchaeal genome reveals new insights into the evolution of the Archaea." Elkins J.G., Podar M., Graham D.E., Makarova K.S., Wolf Y., Randau L., Hedlund B.P., Brochier-Armanet C., Kunin V., Anderson I., Lapidus A., Goltsman E., Barry K., Koonin E.V., Hugenholtz P., Kyrpides N., Wanner G., Richardson P., Keller M., Stetter K.O. Proc. Natl. Acad. Sci. U.S.A. 105:8102-8107(2008) [PubMed: 18535141] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OPF8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000968 Genomic DNA. Translation: ACB07037.1.
RefSeq	YP_001736720.1. NC_010482.1.
3D structure databases
ProteinModelPortal	B1L3K8.
ModBase	Search...
Protein-protein interaction databases
STRING	B1L3K8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6093567.
GenomeReviews	Gene locus Kcr_0279 in contig CP000968_GR.
KEGG	kcr:Kcr_0279.
Phylogenomic databases
HOGENOM	HBG570821.
OMA	GRPRPFQ.
PhylomeDB	B1L3K8.
Enzyme and pathway databases
BioCyc	CKOR374847:KCR_0279-MONOMER.
Family and domain databases
HAMAP	MF_00407. DNA_ligase. [Tree]
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:1.10.3260.10. DNA_ligase_ATP-dep_N. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K10747.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI1_KORCO

Accession

Primary (citable) accession number: B1L3K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	April 29, 2008
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program