B1L1B2 (B1L1B2_CLOBM) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase HAMAP MF_00555
EC=6.3.1.1 HAMAP MF_00555

Alternative name(s):
Asparagine synthetase A HAMAP MF_00555

Gene names

Name:	asnA HAMAP MF_00555 EMBL ACA54248.1
Ordered Locus Names:	CLK_0983

Organism

Clostridium botulinum (strain Loch Maree / Type A3) [Complete proteome] [HAMAP]

Taxonomic identifier

498214 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555 SAAS SAAS004618
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555 SAAS SAAS004618
Subcellular location	Cytoplasm By similarity HAMAP MF_00555 SAAS SAAS004618.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily. HAMAP MF_00555

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis HAMAP MF_00555 SAAS SAAS004618
Cellular component	Cytoplasm HAMAP MF_00555 SAAS SAAS004618
Ligand	ATP-binding HAMAP MF_00555 SAAS SAAS004618 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00555 SAAS SAAS004618 EMBL ACA54248.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B1L1B2 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 250AA1E1089EBFD9	FASTA	340	39,521
	10 20 30 40 50 60 MIKFKDRLMI PEGYEATLGI RETEVAIKEV KDFFERTLAE KLNLTRVSAP LFVRKNTGMN 70 80 90 100 110 120 DNLNGVERPV AFDMKDLQDE MIEIVHSLAK WKRMALHRYD FKVGEGLYTD MNAIRRDEDL 130 140 150 160 170 180 DNLHSIYVDQ WDWERVIKKE DRNKETLKAI VNKIYSAFKE TERYICSQYE GFNEILPEEI 190 200 210 220 230 240 CFITSQELQD TYPDLDSKER EDAITKEKGA VFLMEIGGVL ASGEKHDGRA PDYDDWTLNG 250 260 270 280 290 300 DILFWNPVLE RAFELSSMGI RVDEEALEKQ LKITGCEDRK NLEFHRLLLE GKLPYTVGGG 310 320 330 340 IGQSRICMYF LRKAHIGEVQ ASIWPDLMIE DCEKAKIKLL

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000962 Genomic DNA. Translation: ACA54248.1.
RefSeq	YP_001786925.1. NC_010520.1.
3D structure databases
ProteinModelPortal	B1L1B2.
SMR	B1L1B2. Positions 21-334.
ModBase	Search...
Protein-protein interaction databases
STRING	B1L1B2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6151344.
GenomeReviews	Gene locus CLK_0983 in contig CP000962_GR.
KEGG	cbl:CLK_0983.
PATRIC	19388580. VBICloBot822_1903.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B1L1B2_CLOBM

Accession

Primary (citable) accession number: B1L1B2

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)