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Protein

Ribosomal RNA small subunit methyltransferase H

Gene

rsmH

Organism
Clostridium botulinum (strain Loch Maree / Type A3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53S-adenosyl-L-methionineUniRule annotation1
Binding sitei79S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei100S-adenosyl-L-methionineUniRule annotation1
Binding sitei107S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase HUniRule annotation (EC:2.1.1.199UniRule annotation)
Alternative name(s):
16S rRNA m(4)C1402 methyltransferaseUniRule annotation
rRNA (cytosine-N(4)-)-methyltransferase RsmHUniRule annotation
Gene namesi
Name:rsmHUniRule annotation
Synonyms:mraW
Ordered Locus Names:CLK_0935
OrganismiClostridium botulinum (strain Loch Maree / Type A3)
Taxonomic identifieri498214 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000722 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003868171 – 309Ribosomal RNA small subunit methyltransferase HAdd BLAST309

Structurei

3D structure databases

ProteinModelPortaliB1L164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 35S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RsmH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000049777.
KOiK03438.
OMAiHVPVMLQ.
OrthoDBiPOG091H02LX.

Family and domain databases

Gene3Di1.10.150.170. 1 hit.
3.40.50.150. 2 hits.
HAMAPiMF_01007. 16SrRNA_methyltr_H. 1 hit.
InterProiIPR002903. RsmH.
IPR023397. SAM-dep_MeTrfase_MraW_recog.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11265. PTHR11265. 1 hit.
PfamiPF01795. Methyltransf_5. 1 hit.
[Graphical view]
PIRSFiPIRSF004486. MraW. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
SSF81799. SSF81799. 1 hit.
TIGRFAMsiTIGR00006. TIGR00006. 1 hit.

Sequencei

Sequence statusi: Complete.

B1L164-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFKHISVLL EETIDSLNIK EDGVYVDCTL GGGGHSKEIL KKLSHKGKLI
60 70 80 90 100
GIDQDTSAIK AAKERLKDYE NVIYVHNNFY NIDSILEELD IDKVDGIIMD
110 120 130 140 150
LGVSSYQLDE ASRGFSYMKD APLDMRMNRE ENFSAYNVVN SYEEEELFRI
160 170 180 190 200
FKNYGEEKFS RKIARFIVEK RTENPIETTG ELVEIIRKAI PAKFQREGHP
210 220 230 240 250
AKRTFQAIRI EVNKELQILN KAIEDSVNRL NKDGRLSIIT FHSLEDRIVK
260 270 280 290 300
VKFKELEKPC TCPPSFPICV CGKEPQIKII TKKPIEPSKE EKEINSRSRS

AKLRVCRKI
Length:309
Mass (Da):35,603
Last modified:April 29, 2008 - v1
Checksum:iFE1B164E0DE6D220
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000962 Genomic DNA. Translation: ACA54936.1.
RefSeqiWP_012342976.1. NC_010520.1.

Genome annotation databases

EnsemblBacteriaiACA54936; ACA54936; CLK_0935.
KEGGicbl:CLK_0935.
PATRICi19388484. VBICloBot822_1855.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000962 Genomic DNA. Translation: ACA54936.1.
RefSeqiWP_012342976.1. NC_010520.1.

3D structure databases

ProteinModelPortaliB1L164.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA54936; ACA54936; CLK_0935.
KEGGicbl:CLK_0935.
PATRICi19388484. VBICloBot822_1855.

Phylogenomic databases

HOGENOMiHOG000049777.
KOiK03438.
OMAiHVPVMLQ.
OrthoDBiPOG091H02LX.

Family and domain databases

Gene3Di1.10.150.170. 1 hit.
3.40.50.150. 2 hits.
HAMAPiMF_01007. 16SrRNA_methyltr_H. 1 hit.
InterProiIPR002903. RsmH.
IPR023397. SAM-dep_MeTrfase_MraW_recog.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11265. PTHR11265. 1 hit.
PfamiPF01795. Methyltransf_5. 1 hit.
[Graphical view]
PIRSFiPIRSF004486. MraW. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
SSF81799. SSF81799. 1 hit.
TIGRFAMsiTIGR00006. TIGR00006. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSMH_CLOBM
AccessioniPrimary (citable) accession number: B1L164
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: April 29, 2008
Last modified: November 2, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.