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B1KYY3 (GLSA_CLOBM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutaminase

EC=3.5.1.2
Gene names
Name:glsA
Ordered Locus Names:CLK_2195
OrganismClostridium botulinum (strain Loch Maree / Type A3) [Complete proteome] [HAMAP]
Taxonomic identifier498214 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00313

Sequence similarities

Belongs to the glutaminase family.

Ontologies

Keywords
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamine metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionglutaminase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 305305Glutaminase HAMAP-Rule MF_00313
PRO_1000115696

Sites

Binding site611Substrate By similarity
Binding site1131Substrate By similarity
Binding site1581Substrate By similarity
Binding site1651Substrate By similarity
Binding site1891Substrate By similarity
Binding site2411Substrate By similarity
Binding site2591Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
B1KYY3 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: A2698EC8D5B683A9

FASTA30533,256
        10         20         30         40         50         60 
MNRLLKTVIE NNRKWISEGK VASYIPELSK MDKNLLGISV CTLGGEEYWE GDAEVKFTIQ 

        70         80         90        100        110        120 
SISKIVTLML AIIDNGEDYV FSKVGMEPTE TAFNSIVNLE AKESHKPINP MINAGAIVVA 

       130        140        150        160        170        180 
SMVAGKDSDE KFDRILKFTR KISGNNNIDI NLNVYTSEKE TGHRNRALAY FMKSTGALKG 

       190        200        210        220        230        240 
DVEEILDVYF KQCSIEITCK DLARIGVMLA NDGVSPYTGD RIVPRYVARI VKTIMVTCGM 

       250        260        270        280        290        300 
YDASGNFAVH IGIPAKSGVG GGIVACAPRR MGIGVLGTAL DEKGNSIAGT KILEELSKQL 


DLSIF 

« Hide

References

[1]"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Loch Maree / Type A3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000962 Genomic DNA. Translation: ACA56877.1.
RefSeqYP_001788126.1. NC_010520.1.

3D structure databases

ProteinModelPortalB1KYY3.
SMRB1KYY3. Positions 3-305.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING498214.CLK_2195.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA56877; ACA56877; CLK_2195.
GeneID6151943.
KEGGcbl:CLK_2195.
PATRIC19390994. VBICloBot822_3110.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2066.
HOGENOMHOG000216890.
KOK01425.
OMARINALML.
OrthoDBEOG6N94BK.
ProtClustDBPRK00971.

Enzyme and pathway databases

BioCycCBOT498214:GH05-2846-MONOMER.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
HAMAPMF_00313. Glutaminase.
InterProIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERPTHR12544. PTHR12544. 1 hit.
PfamPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMSSF56601. SSF56601. 1 hit.
TIGRFAMsTIGR03814. Gln_ase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLSA_CLOBM
AccessionPrimary (citable) accession number: B1KYY3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: February 19, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families