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B1KYV1

- SYE_CLOBM

UniProt

B1KYV1 - SYE_CLOBM

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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Clostridium botulinum (strain Loch Maree / Type A3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi108 – 1081ZincUniRule annotation
Metal bindingi110 – 1101ZincUniRule annotation
Metal bindingi135 – 1351ZincUniRule annotation
Metal bindingi137 – 1371ZincUniRule annotation
Binding sitei255 – 2551ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCBOT498214:GH05-1199-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:CLK_0537
OrganismiClostridium botulinum (strain Loch Maree / Type A3)
Taxonomic identifieri498214 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000722: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485Glutamate--tRNA ligasePRO_1000090067Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi498214.CLK_0537.

Structurei

3D structure databases

ProteinModelPortaliB1KYV1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionAdd
BLAST
Motifi252 – 2565"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiKLEWYNG.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1KYV1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNKVRTRFA PSPTGYMHVG NLRTALYAYL IAKHDNGDFI LRIEDTDQER
60 70 80 90 100
LVEGALDVIY NTLKITGLSH DEGPDIGGPV GPYVQSERRN IYIEYAEKLI
110 120 130 140 150
EKGEAYYCFC SKERLDMLRA NSEALKRPFR YDKHCIDLSK EEIDKKIAEG
160 170 180 190 200
VPYVIRQKNP TTGSTSFHDE IYGDISVDNS ELDDMILIKS DGLPTYNFAN
210 220 230 240 250
VVDDHLMGIT HVVRGSEYLS SSPKYNRLYE AFGWDVPIYV HCPPIMKDEH
260 270 280 290 300
HKLSKRNGDA SFEDLMAKGY LKEAILNYIA LLGWNPGGEK EVFSMKELIE
310 320 330 340 350
AFNYRNINKA PAVFDTKKLK WMNGEYIRAL SLDKFHEMAL PHYKEALTRD
360 370 380 390 400
LDTKKISELL HTRVEVLNEI PEQLDFFNNL LEYSPEMYIH KKMKTTYENS
410 420 430 440 450
LKSLEEVLPR LEALENWTFE NIKEVCMNLV KELEVKNGVV LWPIRTAVSG
460 470 480
KQFTPGGAFE IADILGKEET LERIKIGINK LKALQ
Length:485
Mass (Da):55,742
Last modified:April 29, 2008 - v1
Checksum:iECCC963755C45EA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000962 Genomic DNA. Translation: ACA53630.1.
RefSeqiYP_001786485.1. NC_010520.1.

Genome annotation databases

EnsemblBacteriaiACA53630; ACA53630; CLK_0537.
GeneIDi6152467.
KEGGicbl:CLK_0537.
PATRICi19387696. VBICloBot822_1461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000962 Genomic DNA. Translation: ACA53630.1 .
RefSeqi YP_001786485.1. NC_010520.1.

3D structure databases

ProteinModelPortali B1KYV1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 498214.CLK_0537.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACA53630 ; ACA53630 ; CLK_0537 .
GeneIDi 6152467.
KEGGi cbl:CLK_0537.
PATRICi 19387696. VBICloBot822_1461.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252720.
KOi K01885.
OMAi KLEWYNG.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci CBOT498214:GH05-1199-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Loch Maree / Type A3.

Entry informationi

Entry nameiSYE_CLOBM
AccessioniPrimary (citable) accession number: B1KYV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: November 26, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3