B1KX59 (B1KX59_CLOBM) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182
EC=2.1.2.9 HAMAP-Rule MF_00182

Gene names

Name:	fmt HAMAP-Rule MF_00182 EMBL ACA56050.1
Ordered Locus Names:	CLK_1891 EMBL ACA56050.1

Organism

Clostridium botulinum (strain Loch Maree / Type A3) [Complete proteome] [HAMAP] EMBL ACA56050.1

Taxonomic identifier

498214 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium botulinum › Clostridium botulinum A

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182 SAAS SAAS001555
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182 SAAS SAAS001555
Sequence similarities	Belongs to the fmt family. HAMAP-Rule MF_00182

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00182 SAAS SAAS001555
Molecular function	Methyltransferase HAMAP-Rule MF_00182 SAAS SAAS001555 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	112 – 115	4	Tetrahydrofolate (THF) binding By similarity HAMAP-Rule MF_00182

Sequences

	Sequence		Length	Mass (Da)
	B1KX59 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 69090B55DC96B298	FASTA	313	34,952
	10 20 30 40 50 60 MKDTTIVFMG TPDFAVPSLK KLIEEFNVKA VFTQPDRPKG RGKKLAMSAV KEVALENNIE 70 80 90 100 110 120 VYQPIRLKND EICIKKLKEI NPDFIIVVAF GQILSKEVLD IPKYGCINLH ASLLPKYRGA 130 140 150 160 170 180 APINWAIIKG ETESGNTTMF MDEGLDTGDM LLKNTVKIED DMTFGELHDI LMETGSKLLV 190 200 210 220 230 240 DTIKGLKEGT IKREKQKSEN IIYASMLNKQ MGKIDWNKTS KEINLLIRGL NPRPVAYTQY 250 260 270 280 290 300 KNEAMKIYNS SILKESSNKT PGTILKVSKE GIKVATGDGI LSITTVQFPG KKPMKVEEYI 310 KGHTIEEGLV LGE

References

[1]

"Analysis of the Neurotoxin Complex Genes in Clostridium botulinum A1-A4 and B1 Strains: BoNT/A3, /Ba4 and /B1 Clusters Are Located within Plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Loch Maree / Type A3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000962 Genomic DNA. Translation: ACA56050.1.
RefSeq	YP_001787824.1. NC_010520.1.
3D structure databases
ProteinModelPortal	B1KX59.
ModBase	Search...
Protein-protein interaction databases
STRING	498214.CLK_1891.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA56050; ACA56050; CLK_1891.
GeneID	6152072.
KEGG	cbl:CLK_1891.
PATRIC	19390380. VBICloBot822_2803.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
OMA	GITLMQM.
ProtClustDB	PRK00005.
Enzyme and pathway databases
BioCyc	CBOT498214:GH05-2469-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR001555. GART_AS. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B1KX59_CLOBM

Accession

Primary (citable) accession number: B1KX59

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)