B1KWP6 (PLSX_CLOBM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphate acyltransferase
EC=2.3.1.n2

Alternative name(s):
Acyl-ACP phosphotransacylase
Acyl-[acyl-carrier-protein]--phosphate acyltransferase
Phosphate-acyl-ACP acyltransferase

Gene names

Name:	plsX
Ordered Locus Names:	CLK_1833

Organism

Clostridium botulinum (strain Loch Maree / Type A3) [Complete proteome] [HAMAP]

Taxonomic identifier

498214 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium botulinum › Clostridium botulinum A

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible formation of acyl-phosphate (acyl-PO4) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA By similarity. HAMAP-Rule MF_00019
Catalytic activity	Acyl-[acyl-carrier-protein] + phosphate = acyl-phosphate + [acyl-carrier-protein]. HAMAP-Rule MF_00019
Pathway	Lipid metabolism; phospholipid metabolism. HAMAP-Rule MF_00019
Subunit structure	Homodimer. Probably interacts with PlsY By similarity.
Subcellular location	Cytoplasm By similarity. Note: Associated with the membrane possibly through PlsY By similarity.
Sequence similarities	Belongs to the PlsX family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Phosphate acyltransferase HAMAP-Rule MF_00019		PRO_1000089894

Sequences

	Sequence		Length	Mass (Da)
	B1KWP6 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: C81DBC771A0A7529	FASTA	335	36,252
	10 20 30 40 50 60 MIIAVDGMGG DFAPELVVEG CIQAVKEYEG IHIIITGKKE LIKNELDKRE YKGNKIEILN 70 80 90 100 110 120 AEEVISTNEA PVKAIRRKKD SSMVKALELV KEGKAQAVIS AGSTGALMAG ATFVLGRIKG 130 140 150 160 170 180 INRVCLAPLL PGAKAPFMIV DAGANVDCKA EYLVQFAMMG KVYFESVLGV KSPTVGLVNI 190 200 210 220 230 240 GAEEEKGNEL TKAAYKLLKD TDFNFIGNIE PRDIPRGEVN IAVCDGFIGN TVLKTYEGVA 250 260 270 280 290 300 SNLFSMLKDE MMASTRGKIG GALLKPVFKD FKKKFDYTEY GGSPFLGAKG ICIKAHGSSD 310 320 330 AKAFKNAIRQ AKICYDKKII EEIENNLGNL IENNI

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Loch Maree / Type A3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000962 Genomic DNA. Translation: ACA55334.1.
RefSeq	YP_001787768.1. NC_010520.1.
3D structure databases
ProteinModelPortal	B1KWP6.
ModBase	Search...
Protein-protein interaction databases
STRING	498214.CLK_1833.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA55334; ACA55334; CLK_1833.
GeneID	6154421.
KEGG	cbl:CLK_1833.
PATRIC	19390268. VBICloBot822_2747.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0416.
HOGENOM	HOG000154731.
KO	K03621.
OMA	DEKPSQA.
ProtClustDB	PRK05331.
Enzyme and pathway databases
BioCyc	CBOT498214:GH05-1751-MONOMER.
UniPathway	UPA00085.
Family and domain databases
Gene3D	3.40.718.10. 1 hit.
HAMAP	MF_00019. PlsX.
InterPro	IPR003664. FA_synthesis. IPR024084. IsoPropMal-DH-like_dom. IPR012281. Phospholipid_synth_PlsX-like. [Graphical view]
Pfam	PF02504. FA_synthesis. 1 hit. [Graphical view]
PIRSF	PIRSF002465. Phsphlp_syn_PlsX. 1 hit.
TIGRFAMs	TIGR00182. plsX. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSX_CLOBM

Accession

Primary (citable) accession number: B1KWP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program