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Protein

Probable glycine dehydrogenase (decarboxylating) subunit 2

Gene

gcvPB

Organism
Clostridium botulinum (strain Loch Maree / Type A3)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.UniRule annotation

Catalytic activityi

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2.UniRule annotationSAAS annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotationSAAS annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationSAAS annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciCBOT498214:GH05-763-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glycine dehydrogenase (decarboxylating) subunit 2UniRule annotation (EC:1.4.4.2UniRule annotation)
Alternative name(s):
Glycine cleavage system P-protein subunit 2UniRule annotation
Glycine decarboxylase subunit 2UniRule annotation
Glycine dehydrogenase (aminomethyl-transferring) subunit 2UniRule annotation
Gene namesi
Name:gcvPBUniRule annotationImported
Ordered Locus Names:CLK_0100Imported
OrganismiClostridium botulinum (strain Loch Maree / Type A3)Imported
Taxonomic identifieri498214 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000722 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei272 – 2721N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliB1KWD8.
SMRiB1KWD8. Positions 6-484.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvP family. C-terminal subunit subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1003.
HOGENOMiHOG000239368.
KOiK00283.
OMAiMHINLHK.
OrthoDBiEOG6HMXDX.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
HAMAPiMF_00713. GcvPB.
InterProiIPR023012. GcvPB.
IPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11773. PTHR11773. 1 hit.
PfamiPF02347. GDC-P. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

B1KWD8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDYNKVIFE LSSEGRKGYR LPDLDVPEVE LSKLLPKNLL REDEIELPEV
60 70 80 90 100
SEVDVVRHYT ALSNKNYTVD NGFYPLGSCT MKYNPKINED IVALSGFSKI
110 120 130 140 150
HPLQDENISQ GALELMYDLK GKLCEITGLD DFTLQPAAGA HGEYTGLLII
160 170 180 190 200
KAYHEKRGDT KRTKIIVPDS AHGTNPASAS VAGFDIVEIK SGEDGRVSIE
210 220 230 240 250
ELKKVLNDEI AGLMLTNPST LGLFEKDIKL ISELVHEAGG LLYYDGANLN
260 270 280 290 300
AIMGIARPGD MGFDVCHLNM HKTFSTPHGG GGPGSGPVGV KKHLAKFLPV
310 320 330 340 350
PTVEKENDKF ILDYNREDSL GKIRSLYGNF GVMVKAYTYI LTMGKEGLKS
360 370 380 390 400
ASENAVLNAN YIKESLRDYY NIGKDDICKH EVILSTLKEN PHHIATLDIA
410 420 430 440 450
KRLIDYGVHP PTVYFPLIIE EALMIEPTES ESKETVDEFI DAMKKIAVEA
460 470 480
KENPELLHEA PVKAPVRRLD QVKAARKPIL RWQK
Length:484
Mass (Da):53,762
Last modified:April 29, 2008 - v1
Checksum:i858671CD922F0D66
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000962 Genomic DNA. Translation: ACA56785.1.
RefSeqiWP_012344606.1. NC_010520.1.

Genome annotation databases

EnsemblBacteriaiACA56785; ACA56785; CLK_0100.
KEGGicbl:CLK_0100.
PATRICi19386837. VBICloBot822_1032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000962 Genomic DNA. Translation: ACA56785.1.
RefSeqiWP_012344606.1. NC_010520.1.

3D structure databases

ProteinModelPortaliB1KWD8.
SMRiB1KWD8. Positions 6-484.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA56785; ACA56785; CLK_0100.
KEGGicbl:CLK_0100.
PATRICi19386837. VBICloBot822_1032.

Phylogenomic databases

eggNOGiCOG1003.
HOGENOMiHOG000239368.
KOiK00283.
OMAiMHINLHK.
OrthoDBiEOG6HMXDX.

Enzyme and pathway databases

BioCyciCBOT498214:GH05-763-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
HAMAPiMF_00713. GcvPB.
InterProiIPR023012. GcvPB.
IPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11773. PTHR11773. 1 hit.
PfamiPF02347. GDC-P. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Loch Maree / Type A3Imported.

Entry informationi

Entry nameiB1KWD8_CLOBM
AccessioniPrimary (citable) accession number: B1KWD8
Entry historyi
Integrated into UniProtKB/TrEMBL: April 29, 2008
Last sequence update: April 29, 2008
Last modified: July 22, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.