B1KWD5 (GCST_CLOBM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	CLK_0097

Organism

Clostridium botulinum (strain Loch Maree / Type A3) [Complete proteome] [HAMAP]

Taxonomic identifier

498214 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Aminomethyltransferase HAMAP MF_00259		PRO_1000114090

Sequences

	Sequence		Length	Mass (Da)
	B1KWD5 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 8798B8380EBCE64C	FASTA	370	41,553
	10 20 30 40 50 60 MEGLKVTPLR GVYEEYGGKI VDFAGYELPT QFKGFLHEHH TVREKAGLFD VSHMGEAMVT 70 80 90 100 110 120 GKDAGKFIQY LMTNDINVLK DNEVLYTFMC NEDGGVIDDL LVYKFAEDEF FLVINASNKD 130 140 150 160 170 180 KDVKWIMDHK GDFDVEIADV SDSIAQLALQ GPLAEEILQK IVDVDLQEIK FFKLRRDVLV 190 200 210 220 230 240 DGKKCLVSRT GYTGEDGFEI YCKPEDAKGL WHAILNAGKE EGAQPIGLGA RDTLRFEASL 250 260 270 280 290 300 LLYGNEMDET ITPLEVGMGF FVKLKVEEDF IGKDALIKQK AEGVTRKLVG FELIDKGIPR 310 320 330 340 350 360 HGYEVIKDGK VIGHVTTGYK SPTLNKAIGL ALVEEQYSKI GTEFNIKVRK KELKAVAIDK 370 RFYTKKTKTK

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Loch Maree / Type A3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000962 Genomic DNA. Translation: ACA55890.1.
RefSeq	YP_001786048.1. NC_010520.1.
3D structure databases
ProteinModelPortal	B1KWD5.
SMR	B1KWD5. Positions 4-362.
ModBase	Search...
Protein-protein interaction databases
STRING	B1KWD5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6152135.
GenomeReviews	Gene locus CLK_0097 in contig CP000962_GR.
KEGG	cbl:CLK_0097.
PATRIC	19386831. VBICloBot822_1029.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG299834.
OMA	VINASNK.
ProtClustDB	PRK00389.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. [Graphical view]
KO	K00605.
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. GcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_CLOBM

Accession

Primary (citable) accession number: B1KWD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program