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B1KW01 (THIM1_CLOBM) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase 1

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase 1
Short name=TH kinase 1
Short name=Thz kinase 1
Gene names
Name:thiM1
Ordered Locus Names:CLK_1695
OrganismClostridium botulinum (strain Loch Maree / Type A3) [Complete proteome] [HAMAP]
Taxonomic identifier498214 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length265 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 265265Hydroxyethylthiazole kinase 1 HAMAP-Rule MF_00228
PRO_0000383846

Sites

Binding site391Substrate; via amide nitrogen By similarity
Binding site1151ATP By similarity
Binding site1681ATP By similarity
Binding site1951Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
B1KW01 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 9763331C39399774

FASTA26528,747
        10         20         30         40         50         60 
MQIRQSVKFK KPLIHYITNP ISINDCANMI LAVGAKPIMA EHPLEVSEIT SISESLGINL 

        70         80         90        100        110        120 
GNITDNKMKS MLISGKTSYE KKIPQVIDLV GVGCSKLRLD YAKKFISECH PNVIKGNMSE 

       130        140        150        160        170        180 
IKAIYGIKSS AKGIDVGECD IITEQNFDEN IEMIKRLSME TDSVVAATGV VDIISNGTYT 

       190        200        210        220        230        240 
YIISNGCEML SMITGTGSML TGIIASYISS GNILEGTALA IAIMGICGEL SQNVKGTGSF 

       250        260 
RNELIDNMFS ISDDIIIKKI RINSY 

« Hide

References

[1]"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Loch Maree / Type A3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000962 Genomic DNA. Translation: ACA54005.1.
RefSeqYP_001787630.1. NC_010520.1.

3D structure databases

ProteinModelPortalB1KW01.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING498214.CLK_1695.

Proteomic databases

PRIDEB1KW01.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA54005; ACA54005; CLK_1695.
GeneID6153693.
KEGGcbl:CLK_1695.
PATRIC19389994. VBICloBot822_2610.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMAAKPIMAE.
OrthoDBEOG628F8M.

Enzyme and pathway databases

BioCycCBOT498214:GH05-2350-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
SUPFAMSSF53613. SSF53613. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM1_CLOBM
AccessionPrimary (citable) accession number: B1KW01
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: April 29, 2008
Last modified: June 11, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways