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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Clostridium botulinum (strain Loch Maree / Type A3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway:iN(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431S-adenosyl-L-methionineUniRule annotation
Binding sitei68 – 681S-adenosyl-L-methionineUniRule annotation
Binding sitei101 – 1011S-adenosyl-L-methionineUniRule annotation
Binding sitei123 – 1231S-adenosyl-L-methionineUniRule annotation
Binding sitei127 – 1271SubstrateUniRule annotation
Binding sitei159 – 1591SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBOT498214:GH05-558-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:CLK_3710
OrganismiClostridium botulinum (strain Loch Maree / Type A3)
Taxonomic identifieri498214 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000722 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217tRNA (guanine-N(7)-)-methyltransferasePRO_1000136349Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB1KV69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1346Interaction with RNAUniRule annotation
Regioni196 – 1994Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiAHPEINY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1KV69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRKKWWAR PEIEASDKFA DEPKELRGKW NKEFNNNNDI HLELGCGRGG
60 70 80 90 100
FISQLVEKNK DINYVGIDLK DEVIVYAIRK VKEKEEEVKR EFKNIKFVTM
110 120 130 140 150
NIMGIAEVFD KNEISKIYIN FCNPWPKERH NKRRLTHTKL LTEYKKFLKP
160 170 180 190 200
NTEIWFKTDD KELFEDSQEY FKESGFNIEY ITYDLHNSDF KENIKTEYET
210
KFETMGMKIM FLKARLL
Length:217
Mass (Da):26,245
Last modified:April 29, 2008 - v1
Checksum:iA221EAA43064DA26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000962 Genomic DNA. Translation: ACA55013.1.
RefSeqiWP_012343045.1. NC_010520.1.

Genome annotation databases

EnsemblBacteriaiACA55013; ACA55013; CLK_3710.
KEGGicbl:CLK_3710.
PATRICi19386431. VBICloBot822_0829.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000962 Genomic DNA. Translation: ACA55013.1.
RefSeqiWP_012343045.1. NC_010520.1.

3D structure databases

ProteinModelPortaliB1KV69.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA55013; ACA55013; CLK_3710.
KEGGicbl:CLK_3710.
PATRICi19386431. VBICloBot822_0829.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiAHPEINY.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciCBOT498214:GH05-558-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Loch Maree / Type A3.

Entry informationi

Entry nameiTRMB_CLOBM
AccessioniPrimary (citable) accession number: B1KV69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: July 22, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.