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B1KV69 (TRMB_CLOBM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:CLK_3710
OrganismClostridium botulinum (strain Loch Maree / Type A3) [Complete proteome] [HAMAP]
Taxonomic identifier498214 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium ›

Protein attributes

Sequence length217 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 217217tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000136349

Regions

Region129 – 1346Interaction with RNA Potential
Region196 – 1994Substrate binding Potential

Sites

Binding site431S-adenosyl-L-methionine By similarity
Binding site681S-adenosyl-L-methionine By similarity
Binding site1011S-adenosyl-L-methionine By similarity
Binding site1231S-adenosyl-L-methionine By similarity
Binding site1271Substrate By similarity
Binding site1591Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
B1KV69 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: A221EAA43064DA26

FASTA21726,245
        10         20         30         40         50         60 
MRLRKKWWAR PEIEASDKFA DEPKELRGKW NKEFNNNNDI HLELGCGRGG FISQLVEKNK 

        70         80         90        100        110        120 
DINYVGIDLK DEVIVYAIRK VKEKEEEVKR EFKNIKFVTM NIMGIAEVFD KNEISKIYIN 

       130        140        150        160        170        180 
FCNPWPKERH NKRRLTHTKL LTEYKKFLKP NTEIWFKTDD KELFEDSQEY FKESGFNIEY 

       190        200        210 
ITYDLHNSDF KENIKTEYET KFETMGMKIM FLKARLL

« Hide

References

[1]"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Loch Maree / Type A3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000962 Genomic DNA. Translation: ACA55013.1.
RefSeqYP_001785847.1. NC_010520.1.

3D structure databases

ProteinModelPortalB1KV69.
ModBaseSearch...

Protein-protein interaction databases

STRING498214.CLK_3710.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA55013; ACA55013; CLK_3710.
GeneID6153532.
KEGGcbl:CLK_3710.
PATRIC19386431. VBICloBot822_0829.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMARLRNKPW.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycCBOT498214:GH05-1429-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMB_CLOBM
AccessionPrimary (citable) accession number: B1KV69
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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