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B1KV69

- TRMB_CLOBM

UniProt

B1KV69 - TRMB_CLOBM

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene
trmB, CLK_3710
Organism
Clostridium botulinum (strain Loch Maree / Type A3)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431S-adenosyl-L-methionine By similarity
Binding sitei68 – 681S-adenosyl-L-methionine By similarity
Binding sitei101 – 1011S-adenosyl-L-methionine By similarity
Binding sitei123 – 1231S-adenosyl-L-methionine By similarity
Binding sitei127 – 1271Substrate By similarity
Binding sitei159 – 1591Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBOT498214:GH05-558-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Ordered Locus Names:CLK_3710
OrganismiClostridium botulinum (strain Loch Maree / Type A3)
Taxonomic identifieri498214 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000722: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217tRNA (guanine-N(7)-)-methyltransferaseUniRule annotationPRO_1000136349Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi498214.CLK_3710.

Structurei

3D structure databases

ProteinModelPortaliB1KV69.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1346Interaction with RNA Reviewed prediction
Regioni196 – 1994Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1KV69-1 [UniParc]FASTAAdd to Basket

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MRLRKKWWAR PEIEASDKFA DEPKELRGKW NKEFNNNNDI HLELGCGRGG    50
FISQLVEKNK DINYVGIDLK DEVIVYAIRK VKEKEEEVKR EFKNIKFVTM 100
NIMGIAEVFD KNEISKIYIN FCNPWPKERH NKRRLTHTKL LTEYKKFLKP 150
NTEIWFKTDD KELFEDSQEY FKESGFNIEY ITYDLHNSDF KENIKTEYET 200
KFETMGMKIM FLKARLL 217
Length:217
Mass (Da):26,245
Last modified:April 29, 2008 - v1
Checksum:iA221EAA43064DA26
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000962 Genomic DNA. Translation: ACA55013.1.
RefSeqiYP_001785847.1. NC_010520.1.

Genome annotation databases

EnsemblBacteriaiACA55013; ACA55013; CLK_3710.
GeneIDi6153532.
KEGGicbl:CLK_3710.
PATRICi19386431. VBICloBot822_0829.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000962 Genomic DNA. Translation: ACA55013.1 .
RefSeqi YP_001785847.1. NC_010520.1.

3D structure databases

ProteinModelPortali B1KV69.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 498214.CLK_3710.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACA55013 ; ACA55013 ; CLK_3710 .
GeneIDi 6153532.
KEGGi cbl:CLK_3710.
PATRICi 19386431. VBICloBot822_0829.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000251689.
KOi K03439.
OMAi DNVMTEY.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci CBOT498214:GH05-558-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Loch Maree / Type A3.

Entry informationi

Entry nameiTRMB_CLOBM
AccessioniPrimary (citable) accession number: B1KV69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: June 11, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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