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Protein

Alanine racemase

Gene

alr

Organism
Clostridium botulinum (strain Loch Maree / Type A3)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

Catalytic activityi

L-alanine = D-alanine.UniRule annotationSAAS annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotationSAAS annotation

Pathway:iD-alanine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes D-alanine from L-alanine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine racemase (alr), Alanine racemase (alr), Alanine racemase (alr)
This subpathway is part of the pathway D-alanine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-alanine from L-alanine, the pathway D-alanine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei38 – 381Proton acceptor; specific for D-alanineUniRule annotation
Binding sitei144 – 1441SubstrateUniRule annotation
Active sitei275 – 2751Proton acceptor; specific for L-alanineUniRule annotation
Binding sitei328 – 3281Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotationSAAS annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciCBOT498214:GH05-316-MONOMER.
UniPathwayiUPA00042; UER00497.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemaseUniRule annotation (EC:5.1.1.1UniRule annotation)
Gene namesi
Name:alrImported
Ordered Locus Names:CLK_3463Imported
OrganismiClostridium botulinum (strain Loch Maree / Type A3)Imported
Taxonomic identifieri498214 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000722 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei38 – 381N6-(pyridoxal phosphate)lysineUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB1KTP9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031444.
KOiK01775.
OMAiFDLGTKG.
OrthoDBiEOG6PP9NJ.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1KTP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEHSRPAWI EINLDNLIHN MKEIRRVARS KEIISVVKAD AYGHGALEIA
60 70 80 90 100
SLLLENGANR LAVAVLSEAL ELRNNGITAP IIILGYTPLR YDEKTICNNA
110 120 130 140 150
ETIIKNDLEV SVSSYKYAEQ LSKKAQQLGK DVKIHVNIDT GMGRLGFLPT
160 170 180 190 200
EENLEKIYQI SKLPNIIFEG LFSHFSTADE KNKDYTNEQF KKFLDFYYKL
210 220 230 240 250
RDKNVKVNIK HIANSAALID LPYTHLDAVR PGIAQYGYYP SSEVNHKDIN
260 270 280 290 300
LRPIMTLKTN IVLVKDIKSG QSISYGRKFR TIRNSKIAVL PIGYADGYER
310 320 330 340 350
ELSNENNVYK AQVIVNGRRA PIVGRITMDM CMIDVTDIGD VKVGDEVILI
360 370 380 390 400
GECNDERISV EDIADLLNTI PYEVTSRISK RIPRYYMKDG KIVKISQINK

EVNK
Length:404
Mass (Da):45,827
Last modified:April 29, 2008 - v1
Checksum:i08F9BC365E395B72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000962 Genomic DNA. Translation: ACA53599.1.
RefSeqiWP_012341804.1. NC_010520.1.

Genome annotation databases

EnsemblBacteriaiACA53599; ACA53599; CLK_3463.
KEGGicbl:CLK_3463.
PATRICi19385945. VBICloBot822_0593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000962 Genomic DNA. Translation: ACA53599.1.
RefSeqiWP_012341804.1. NC_010520.1.

3D structure databases

ProteinModelPortaliB1KTP9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA53599; ACA53599; CLK_3463.
KEGGicbl:CLK_3463.
PATRICi19385945. VBICloBot822_0593.

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031444.
KOiK01775.
OMAiFDLGTKG.
OrthoDBiEOG6PP9NJ.

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.
BioCyciCBOT498214:GH05-316-MONOMER.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Loch Maree / Type A3Imported.

Entry informationi

Entry nameiB1KTP9_CLOBM
AccessioniPrimary (citable) accession number: B1KTP9
Entry historyi
Integrated into UniProtKB/TrEMBL: April 29, 2008
Last sequence update: April 29, 2008
Last modified: July 22, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.