B1KTJ6 (TPIS_CLOBM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	CLK_3410

Organism

Clostridium botulinum (strain Loch Maree / Type A3) [Complete proteome] [HAMAP]

Taxonomic identifier

498214 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium botulinum › Clostridium botulinum A

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP-Rule MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP-Rule MF_00147
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00147
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00147.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Triosephosphate isomerase HAMAP-Rule MF_00147		PRO_1000096489
Sites
	Active site	94	1	Electrophile By similarity
	Active site	166	1	Proton acceptor By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	11	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1KTJ6 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 26FC0B31EAE7BBF6	FASTA	248	27,337
	10 20 30 40 50 60 MRTAIIAGNW KMNKTVKEAV ELVKELKPLV KDAKCDVVVC PTYVCLPAVL EEVKGSNIKV 70 80 90 100 110 120 GAQNMHFEES GAYTGEISPK MLEELGVHYV IIGHSERRQY FNETDETVNK KVKKAFEHNL 130 140 150 160 170 180 IPIVCCGESL EQREGNVTEK VLEGQIKVDL KELSKEQVEK LVIAYEPIWA IGTGKTATDE 190 200 210 220 230 240 QANETIGYIR TVVKSMYGED VASKVRIQYG GSVKPGTIKA QMAKEEIDGA LVGGASLKAE DFSAIVNF

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Loch Maree / Type A3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000962 Genomic DNA. Translation: ACA56414.1.
RefSeq	YP_001785559.1. NC_010520.1.
3D structure databases
ProteinModelPortal	B1KTJ6.
SMR	B1KTJ6. Positions 2-248.
ModBase	Search...
Protein-protein interaction databases
STRING	498214.CLK_3410.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA56414; ACA56414; CLK_3410.
GeneID	6153595.
KEGG	cbl:CLK_3410.
PATRIC	19385839. VBICloBot822_0540.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0149.
HOGENOM	HOG000226413.
KO	K01803.
OMA	ANTVIAY.
ProtClustDB	PRK00042.
Enzyme and pathway databases
BioCyc	CBOT498214:GH05-263-MONOMER.
UniPathway	UPA00109; UER00189. UPA00138.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00147_B. TIM_B.
InterPro	IPR013785. Aldolase_TIM. IPR022896. TrioseP_Isoase_bac/euk. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
PANTHER	PTHR21139. PTHR21139. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_CLOBM

Accession

Primary (citable) accession number: B1KTJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	May 29, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program