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Protein

Triosephosphate isomerase

Gene

tpiA

Organism
Clostridium botulinum (strain Loch Maree / Type A3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).UniRule annotation

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (tpiA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei94ElectrophileUniRule annotation1
Active sitei166Proton acceptorUniRule annotation1
Binding sitei172Substrate; via amide nitrogenUniRule annotation1
Binding sitei212SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processGluconeogenesis, Glycolysis

Enzyme and pathway databases

UniPathwayiUPA00109; UER00189
UPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomeraseUniRule annotation (EC:5.3.1.1UniRule annotation)
Short name:
TIMUniRule annotation
Short name:
TPIUniRule annotation
Alternative name(s):
Triose-phosphate isomeraseUniRule annotation
Gene namesi
Name:tpiAUniRule annotation
Ordered Locus Names:CLK_3410
OrganismiClostridium botulinum (strain Loch Maree / Type A3)
Taxonomic identifieri498214 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000722 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000964891 – 248Triosephosphate isomeraseAdd BLAST248

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB1KTJ6
SMRiB1KTJ6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni9 – 11Substrate bindingUniRule annotation3
Regioni233 – 234Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000226413
KOiK01803
OMAiLCVGEGL
OrthoDBiPOG091H02GL

Family and domain databases

CDDicd00311 TIM, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00147_B TIM_B, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR035990 TIM_sf
IPR022896 TrioseP_Isoase_bac/euk
IPR000652 Triosephosphate_isomerase
IPR020861 Triosephosphate_isomerase_AS
PANTHERiPTHR21139 PTHR21139, 1 hit
PfamiView protein in Pfam
PF00121 TIM, 1 hit
SUPFAMiSSF51351 SSF51351, 1 hit
TIGRFAMsiTIGR00419 tim, 1 hit
PROSITEiView protein in PROSITE
PS00171 TIM_1, 1 hit
PS51440 TIM_2, 1 hit

Sequencei

Sequence statusi: Complete.

B1KTJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTAIIAGNW KMNKTVKEAV ELVKELKPLV KDAKCDVVVC PTYVCLPAVL
60 70 80 90 100
EEVKGSNIKV GAQNMHFEES GAYTGEISPK MLEELGVHYV IIGHSERRQY
110 120 130 140 150
FNETDETVNK KVKKAFEHNL IPIVCCGESL EQREGNVTEK VLEGQIKVDL
160 170 180 190 200
KELSKEQVEK LVIAYEPIWA IGTGKTATDE QANETIGYIR TVVKSMYGED
210 220 230 240
VASKVRIQYG GSVKPGTIKA QMAKEEIDGA LVGGASLKAE DFSAIVNF
Length:248
Mass (Da):27,337
Last modified:April 29, 2008 - v1
Checksum:i26FC0B31EAE7BBF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000962 Genomic DNA Translation: ACA56414.1
RefSeqiWP_012344289.1, NC_010520.1

Genome annotation databases

EnsemblBacteriaiACA56414; ACA56414; CLK_3410
KEGGicbl:CLK_3410

Similar proteinsi

Entry informationi

Entry nameiTPIS_CLOBM
AccessioniPrimary (citable) accession number: B1KTJ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: April 25, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health