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B1KTJ6 (TPIS_CLOBM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Triosephosphate isomerase

Short name=TIM
EC=5.3.1.1
Alternative name(s):
Triose-phosphate isomerase
Gene names
Name:tpiA
Ordered Locus Names:CLK_3410
OrganismClostridium botulinum (strain Loch Maree / Type A3) [Complete proteome] [HAMAP]
Taxonomic identifier498214 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP-Rule MF_00147

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00147

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP-Rule MF_00147

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00147

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00147.

Sequence similarities

Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
   Biological processGluconeogenesis
Glycolysis
Pentose shunt
   Cellular componentCytoplasm
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glycolysis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

pentose-phosphate shunt

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontriose-phosphate isomerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 248248Triosephosphate isomerase HAMAP-Rule MF_00147
PRO_1000096489

Sites

Active site941Electrophile By similarity
Active site1661Proton acceptor By similarity
Binding site91Substrate By similarity
Binding site111Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B1KTJ6 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 26FC0B31EAE7BBF6

FASTA24827,337
        10         20         30         40         50         60 
MRTAIIAGNW KMNKTVKEAV ELVKELKPLV KDAKCDVVVC PTYVCLPAVL EEVKGSNIKV 

        70         80         90        100        110        120 
GAQNMHFEES GAYTGEISPK MLEELGVHYV IIGHSERRQY FNETDETVNK KVKKAFEHNL 

       130        140        150        160        170        180 
IPIVCCGESL EQREGNVTEK VLEGQIKVDL KELSKEQVEK LVIAYEPIWA IGTGKTATDE 

       190        200        210        220        230        240 
QANETIGYIR TVVKSMYGED VASKVRIQYG GSVKPGTIKA QMAKEEIDGA LVGGASLKAE 


DFSAIVNF 

« Hide

References

[1]"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Loch Maree / Type A3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000962 Genomic DNA. Translation: ACA56414.1.
RefSeqYP_001785559.1. NC_010520.1.

3D structure databases

ProteinModelPortalB1KTJ6.
SMRB1KTJ6. Positions 2-248.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING498214.CLK_3410.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA56414; ACA56414; CLK_3410.
GeneID6153595.
KEGGcbl:CLK_3410.
PATRIC19385839. VBICloBot822_0540.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0149.
HOGENOMHOG000226413.
KOK01803.
OMAAKLGCTF.
OrthoDBEOG66QM23.
ProtClustDBPRK00042.

Enzyme and pathway databases

BioCycCBOT498214:GH05-263-MONOMER.
UniPathwayUPA00109; UER00189.
UPA00138.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00147_B. TIM_B.
InterProIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERPTHR21139. PTHR21139. 1 hit.
PfamPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMSSF51351. SSF51351. 1 hit.
TIGRFAMsTIGR00419. tim. 1 hit.
PROSITEPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTPIS_CLOBM
AccessionPrimary (citable) accession number: B1KTJ6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: February 19, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways