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Protein

Triosephosphate isomerase

Gene

tpiA

Organism
Clostridium botulinum (strain Loch Maree / Type A3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91SubstrateUniRule annotation
Binding sitei11 – 111SubstrateUniRule annotation
Active sitei94 – 941ElectrophileUniRule annotation
Active sitei166 – 1661Proton acceptorUniRule annotation

GO - Molecular functioni

  1. triose-phosphate isomerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
  2. glycolytic process Source: UniProtKB-HAMAP
  3. pentose-phosphate shunt Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis, Pentose shunt

Enzyme and pathway databases

BioCyciCBOT498214:GH05-263-MONOMER.
UniPathwayiUPA00109; UER00189.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomeraseUniRule annotation (EC:5.3.1.1UniRule annotation)
Short name:
TIMUniRule annotation
Alternative name(s):
Triose-phosphate isomeraseUniRule annotation
Gene namesi
Name:tpiAUniRule annotation
Ordered Locus Names:CLK_3410
OrganismiClostridium botulinum (strain Loch Maree / Type A3)
Taxonomic identifieri498214 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000722: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248Triosephosphate isomerasePRO_1000096489Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi498214.CLK_3410.

Structurei

3D structure databases

ProteinModelPortaliB1KTJ6.
SMRiB1KTJ6. Positions 2-248.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0149.
HOGENOMiHOG000226413.
KOiK01803.
OMAiIEKNGTM.
OrthoDBiEOG66QM23.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1KTJ6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRTAIIAGNW KMNKTVKEAV ELVKELKPLV KDAKCDVVVC PTYVCLPAVL
60 70 80 90 100
EEVKGSNIKV GAQNMHFEES GAYTGEISPK MLEELGVHYV IIGHSERRQY
110 120 130 140 150
FNETDETVNK KVKKAFEHNL IPIVCCGESL EQREGNVTEK VLEGQIKVDL
160 170 180 190 200
KELSKEQVEK LVIAYEPIWA IGTGKTATDE QANETIGYIR TVVKSMYGED
210 220 230 240
VASKVRIQYG GSVKPGTIKA QMAKEEIDGA LVGGASLKAE DFSAIVNF
Length:248
Mass (Da):27,337
Last modified:April 29, 2008 - v1
Checksum:i26FC0B31EAE7BBF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000962 Genomic DNA. Translation: ACA56414.1.
RefSeqiYP_001785559.1. NC_010520.1.

Genome annotation databases

EnsemblBacteriaiACA56414; ACA56414; CLK_3410.
GeneIDi6153595.
KEGGicbl:CLK_3410.
PATRICi19385839. VBICloBot822_0540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000962 Genomic DNA. Translation: ACA56414.1.
RefSeqiYP_001785559.1. NC_010520.1.

3D structure databases

ProteinModelPortaliB1KTJ6.
SMRiB1KTJ6. Positions 2-248.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi498214.CLK_3410.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA56414; ACA56414; CLK_3410.
GeneIDi6153595.
KEGGicbl:CLK_3410.
PATRICi19385839. VBICloBot822_0540.

Phylogenomic databases

eggNOGiCOG0149.
HOGENOMiHOG000226413.
KOiK01803.
OMAiIEKNGTM.
OrthoDBiEOG66QM23.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00189.
UPA00138.
BioCyciCBOT498214:GH05-263-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Loch Maree / Type A3.

Entry informationi

Entry nameiTPIS_CLOBM
AccessioniPrimary (citable) accession number: B1KTJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: January 7, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.