Reviewed, UniProtKB/Swiss-Prot B1KTG4 (MURI_CLOBM)

Names and origin

Protein names

Recommended name:
    Glutamate racemase
    EC=5.1.1.3

Gene names

Name:	murI
Ordered Locus Names:	CLK_3032

Organism

Clostridium botulinum (strain Loch Maree / Type A3) [Complete proteome] [HAMAP]

Taxonomic identifier

498214 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the (R)-glutamate required for cell wall biosynthesis By similarity.
Catalytic activity	L-glutamate = D-glutamate. HAMAP MF_00258
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00258
Sequence similarities	Belongs to the aspartate/glutamate racemases family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glutamate racemase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Glutamate racemase HAMAP MF_00258		PRO_1000114039

Sequences

	Sequence		Length	Mass (Da)
	B1KTG4-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: D645D1D4D7CC1D97	FASTA	257	28,908
	10 20 30 40 50 60 MNINDKPIGF FDSGVGGISV LKEAFKLLPK EDFLYYGDSK NAPYGTKKVE EVKTLTFNAT 70 80 90 100 110 120 DFLMNKGIKA LVVACNTATS VTINDLRENY DIPIIGIEPA LKPAVELKKG GKIIIMATPM 130 140 150 160 170 180 TLAEKKFANL MDLYKETEDI EPLPCPGLPE LIEQGIVSGD VIYNYLKDKF SKYDNEKISS 190 200 210 220 230 240 IVLGCTHYPF IEETLKEVTH NKACIIDGSF GTSRELKRQL KNSNMLREEN RVGKVTIFNS 250 REDRDIIDLS YKLFNMK

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000962 Genomic DNA. Translation: ACA53721.1.
RefSeq	YP_001788902.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6154797.
GenomeReviews	Gene locus CLK_3032 in contig CP000962_GR.
KEGG	cbl:CLK_3032.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	AIWATPA.
Family and domain databases
HAMAP	MF_00258. [Tree]
InterPro	IPR015942. Asp/Glu/hydantoin_racemase. IPR001920. Asp/Glu_race. IPR018187. Asp/Glu_racemase_AS. IPR004391. Glu_race. [Graphical view]
Gene3D	G3DSA:3.40.50.1860. Asp/Glu_race. 1 hit.
Pfam	PF01177. Asp_Glu_race. 1 hit. [Graphical view]
TIGRFAMs	TIGR00067. glut_race. 1 hit.
PROSITE	PS00923. ASP_GLU_RACEMASE_1. 1 hit. PS00924. ASP_GLU_RACEMASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURI_CLOBM

Accession

Primary (citable) accession number: B1KTG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)