Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

B1KT47

- NADK_CLOBM

UniProt

B1KT47 - NADK_CLOBM

Protein

NAD kinase

Gene

nadK

Organism
Clostridium botulinum (strain Loch Maree / Type A3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

    Catalytic activityi

    ATP + NAD+ = ADP + NADP+.UniRule annotation

    Cofactori

    Divalent metal ions.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei61 – 611Proton acceptorUniRule annotation
    Binding sitei145 – 1451NADUniRule annotation
    Binding sitei164 – 1641NADUniRule annotation
    Binding sitei234 – 2341NADUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi61 – 622NADUniRule annotation
    Nucleotide bindingi134 – 1352NADUniRule annotation
    Nucleotide bindingi175 – 1806NADUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-HAMAP
    3. NAD+ kinase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. NAD metabolic process Source: InterPro
    2. NADP biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, NAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCBOT498214:GH05-1925-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
    Alternative name(s):
    ATP-dependent NAD kinaseUniRule annotation
    Gene namesi
    Name:nadKUniRule annotation
    Ordered Locus Names:CLK_1269
    OrganismiClostridium botulinum (strain Loch Maree / Type A3)
    Taxonomic identifieri498214 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000000722: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 281281NAD kinasePRO_1000120847Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi498214.CLK_1269.

    Structurei

    3D structure databases

    ProteinModelPortaliB1KT47.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0061.
    HOGENOMiHOG000227222.
    KOiK00858.
    OMAiRWEALCL.
    OrthoDBiEOG6PZXDR.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B1KT47-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNIGININT DKDISRNILD KIFQYIHEEC SEAKIKVFYD SKGLDNEESR    50
    ALDAVMVLGG DGTILGTARA LAKYDVPIFG INRGHLGFLA EIELEDCKKA 100
    IKNLFKGQYK IENRIMLKCD LKGIDKKDDF LALNDIVLTK GNLSRIVKYS 150
    IYVDDVWYTT FVADGVIVAT PTGSTAYSLS AGGPIVYPDL DVLEIAPICP 200
    HSLGIRPILL NGNSKINIRV LKKYEDPVLT IDGQRYKKVT VNEVTISKSE 250
    YKCRLIKFKD KDYFKILRTK ISYRSRECEG E 281
    Length:281
    Mass (Da):31,689
    Last modified:April 29, 2008 - v1
    Checksum:iE83C4D9A4C63A98A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000962 Genomic DNA. Translation: ACA56485.1.
    RefSeqiYP_001787207.1. NC_010520.1.

    Genome annotation databases

    EnsemblBacteriaiACA56485; ACA56485; CLK_1269.
    GeneIDi6153107.
    KEGGicbl:CLK_1269.
    PATRICi19389146. VBICloBot822_2186.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000962 Genomic DNA. Translation: ACA56485.1 .
    RefSeqi YP_001787207.1. NC_010520.1.

    3D structure databases

    ProteinModelPortali B1KT47.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 498214.CLK_1269.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACA56485 ; ACA56485 ; CLK_1269 .
    GeneIDi 6153107.
    KEGGi cbl:CLK_1269.
    PATRICi 19389146. VBICloBot822_2186.

    Phylogenomic databases

    eggNOGi COG0061.
    HOGENOMi HOG000227222.
    KOi K00858.
    OMAi RWEALCL.
    OrthoDBi EOG6PZXDR.

    Enzyme and pathway databases

    BioCyci CBOT498214:GH05-1925-MONOMER.

    Family and domain databases

    Gene3Di 2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPi MF_00361. NAD_kinase.
    InterProi IPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view ]
    PANTHERi PTHR20275. PTHR20275. 1 hit.
    Pfami PF01513. NAD_kinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
      Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
      PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Loch Maree / Type A3.

    Entry informationi

    Entry nameiNADK_CLOBM
    AccessioniPrimary (citable) accession number: B1KT47
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3