B1KS25 (KHSE_CLOBM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	CLK_1103

Organism

Clostridium botulinum (strain Loch Maree / Type A3) [Complete proteome] [HAMAP]

Taxonomic identifier

498214 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Homoserine kinase HAMAP MF_00384		PRO_1000122413
Regions
	Nucleotide binding	82 – 92	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B1KS25 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 5BB261BA72E08BF4	FASTA	297	32,724
	10 20 30 40 50 60 MVEVRVPATS ANIGPGFDCL GVAVNMYNKF FVEEIEEGLI FEGCADKFKN ENNLIYVAMK 70 80 90 100 110 120 KCFNKIGYKP TGLRIKIESD IPVSRGLGSS AACVVGGIVS ANELAGGALN KKELLDLAVE 130 140 150 160 170 180 VEGHPDNVNP AFCGGMTASI SDNREVIYSK VKVSEGIKFC ALIPDFTLST EKARAVLPKS 190 200 210 220 230 240 IDYKDGIFNV GRTALMISAL NNGDFHLIKY ACKDKLHQDH RAKLIENFYS IKKQCEKLNS 250 260 270 280 290 LGVFLSGAGP TIMVMLREED KDFSKNIKSF LETLKNKWEV RELKIDKLGT VVNNRKV

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Loch Maree / Type A3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000962 Genomic DNA. Translation: ACA55240.1.
RefSeq	YP_001787043.1. NC_010520.1.
3D structure databases
ProteinModelPortal	B1KS25.
ModBase	Search...
Protein-protein interaction databases
STRING	B1KS25.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6152168.
GenomeReviews	Gene locus CLK_1103 in contig CP000962_GR.
KEGG	cbl:CLK_1103.
PATRIC	19388820. VBICloBot822_2023.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646290.
OMA	NNALMST.
ProtClustDB	PRK01212.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_CLOBM

Accession

Primary (citable) accession number: B1KS25

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program