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B1KML5

- SYE_SHEWM

UniProt

B1KML5 - SYE_SHEWM

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Shewanella woodyi (strain ATCC 51908 / MS32)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi98 – 981ZincUniRule annotation
    Metal bindingi100 – 1001ZincUniRule annotation
    Metal bindingi125 – 1251ZincUniRule annotation
    Metal bindingi127 – 1271ZincUniRule annotation
    Binding sitei239 – 2391ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSWOO392500:GI2C-1761-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:Swoo_1728
    OrganismiShewanella woodyi (strain ATCC 51908 / MS32)
    Taxonomic identifieri392500 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000002168: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469Glutamate--tRNA ligasePRO_1000090107Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi392500.Swoo_1728.

    Structurei

    3D structure databases

    ProteinModelPortaliB1KML5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi9 – 1911"HIGH" regionAdd
    BLAST
    Motifi236 – 2405"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252722.
    KOiK01885.
    OMAiHYIKELD.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1KML5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVKTRFAPS PTGFLHVGGA RTALYSWLYA RANQGEFVLR IEDTDLERST    50
    PEACAAILEG MEWLNLNWDE GPYYQTKRFD RYNEIIVQML EQGTAYKCYC 100
    SRERIDTMRE EQAAKGEQQK YDGCCRDKAP RDTDEPFVVR FKNPTEGSVV 150
    FDDHVRGHIE FANSTLDDLI IARTEGTPTY NFCVVVDDWD MGITCVVRGE 200
    DHINNTPRQI NILKALGAPV PEYAHVSMIL GDDGAKLSKR HGAVGVMQYR 250
    DDGYLPEALL NYLVRLGWSH GDQEVFSIDE MKQFFSLDDI NKAASAFNTD 300
    KLIWLNQHYI KELDPEYVAS HLEWHMADQN IDTSNGPKLS EVVSALSERA 350
    KTLKELAASS RYFFEDFAEF DETAAKKHLR GVALEPLTLF QSKLAGLNEW 400
    TLEAIHQAIE DTAAELEVGM GKVGMPLRVA VTGAGMSPAV DLTIFLVGKV 450
    RAEQRISKAI EFVANRINS 469
    Length:469
    Mass (Da):52,814
    Last modified:April 29, 2008 - v1
    Checksum:i8DAF7FD932D9B1C9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000961 Genomic DNA. Translation: ACA86013.1.
    RefSeqiWP_012324359.1. NC_010506.1.
    YP_001760108.1. NC_010506.1.

    Genome annotation databases

    EnsemblBacteriaiACA86013; ACA86013; Swoo_1728.
    GeneIDi6115978.
    KEGGiswd:Swoo_1728.
    PATRICi23605821. VBISheWoo126588_1791.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000961 Genomic DNA. Translation: ACA86013.1 .
    RefSeqi WP_012324359.1. NC_010506.1.
    YP_001760108.1. NC_010506.1.

    3D structure databases

    ProteinModelPortali B1KML5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 392500.Swoo_1728.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACA86013 ; ACA86013 ; Swoo_1728 .
    GeneIDi 6115978.
    KEGGi swd:Swoo_1728.
    PATRICi 23605821. VBISheWoo126588_1791.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252722.
    KOi K01885.
    OMAi HYIKELD.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci SWOO392500:GI2C-1761-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51908 / MS32.

    Entry informationi

    Entry nameiSYE_SHEWM
    AccessioniPrimary (citable) accession number: B1KML5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3