B1KM69 (PLSB_SHEWM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	Swoo_0181

Organism

Shewanella woodyi (strain ATCC 51908 / MS32) [Complete proteome] [HAMAP]

Taxonomic identifier

392500 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000123098
Regions
	Motif	308 – 313	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	B1KM69 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 73609910422D31A8	FASTA	807	91,711
	10 20 30 40 50 60 MSKQDSLWFK SLRWLQKKLV HTIVVPQEPF KDLNLDPEKP LVYVMKTESL SDIAALSEIT 70 80 90 100 110 120 GELGLPSPYE PLEVEGRSTP RVVCLEGAKP LMGKRESNEF FLSSFMDLLK AHKESPDLDI 130 140 150 160 170 180 QLVPVSLYWG RTPGKEDDTM KAAVLERENP TWLRKCLMIL FLGRHNFVQF SNSVSIRYMA 190 200 210 220 230 240 DEHGTDKRIA HKLARVARVH FSRQRKVMTG PVLPKRQALF HALINSESLK RAIQEEATSK 250 260 270 280 290 300 KISEVEARAK AMEYLDEIAA DYSDSLVRIA ERFLTWLWNK LYKGINIKGA EQIRQLHHDG 310 320 330 340 350 360 HEIIYVPCHR SHMDYLLLSY ILYYQGMVPP HIAAGINLNF WPAGPMFRRG GAFFIRRSFR 370 380 390 400 410 420 GNKLYTAVFR EYLDQLFTKG YSVEYFTEGG RSRTGRLLAP KTGMIAMTLN SVLRGMERPV 430 440 450 460 470 480 TLVPVYLGYD HVMEVATYHK ELSGKKKKKE SVWQVFGALR KLGNFGQGYV NFGKPITLHS 490 500 510 520 530 540 FLNEQVPDWR EEIASDPEQK PTWLTPVVNT LANQVMTNIN DAAAVSSVTL TSLVLLASEQ 550 560 570 580 590 600 NALERTQLEK QLDLYLKLLK DLPYTSYTSV VEGDGKQLVT QGLELKKLQL DSDPLGDIVS 610 620 630 640 650 660 IDESISIAMT YYRNNIIHLM IIPSLVASCL TQHERITRDE IKAIIADFYP LLEAELFMGV 670 680 690 700 710 720 EDTDKLVEQI LDLFIEQQLI REDTGFSVVE TSINQLLLLA GTVSETLQRY AIIFNLLADF 730 740 750 760 770 780 PEMERSDLEA ESHKLAKRLG ALHGITAPEF YDKNLYRTLS VKLKDLGYLK DNGNKADVER 790 800 VRASANGLLR SSVRQTIVDS VAAERSA

References

[1]

"Complete sequence of Shewanella woodyi ATCC 51908." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Zhao J.-S., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51908 / MS32.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000961 Genomic DNA. Translation: ACA84482.1.
RefSeq	YP_001758577.1. NC_010506.1.
3D structure databases
ProteinModelPortal	B1KM69.
ModBase	Search...
Protein-protein interaction databases
STRING	B1KM69.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6114404.
GenomeReviews	Gene locus Swoo_0181 in contig CP000961_GR.
KEGG	swd:Swoo_0181.
PATRIC	23602559. VBISheWoo126588_0186.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_SHEWM

Accession

Primary (citable) accession number: B1KM69

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program