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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Shewanella woodyi (strain ATCC 51908 / MS32)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation
Active sitei80 – 801NucleophileUniRule annotation
Active sitei207 – 2071UniRule annotation
Active sitei235 – 2351UniRule annotation
Binding sitei235 – 2351SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Biotin biosynthesis

Enzyme and pathway databases

BioCyciSWOO392500:GI2C-162-MONOMER.
UniPathwayiUPA00078.

Protein family/group databases

ESTHERishewm-bioh. BioH.
MEROPSiS33.994.

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
Alternative name(s):
Biotin synthesis protein BioHUniRule annotation
Carboxylesterase BioHUniRule annotation
Gene namesi
Name:bioHUniRule annotation
Ordered Locus Names:Swoo_0156
OrganismiShewanella woodyi (strain ATCC 51908 / MS32)
Taxonomic identifieri392500 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000002168 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256Pimeloyl-[acyl-carrier protein] methyl ester esterasePRO_1000140004Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi392500.Swoo_0156.

Structurei

3D structure databases

ProteinModelPortaliB1KM44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 812Substrate bindingUniRule annotation
Regioni143 – 1475Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3C. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028062.
KOiK02170.
OMAiALMDEQY.
OrthoDBiPOG091H04D0.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.

Sequencei

Sequence statusi: Complete.

B1KM44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSLHIESI GQGQELVILH GWGVNSSVFT PLHASLSEYR VHYVDLPGFG
60 70 80 90 100
HSDPIDGDLD DWVDAIINQL PNTAIWIGWS LGGLVATKAA LRYPEQVRGL
110 120 130 140 150
VTIASSPCFM AREDESWPGI PPQVLSQFST QLQQNIGKTI ERFLAIQVMG
160 170 180 190 200
SATAKEDIKQ LKELVLSRPL PKNSALAQGL KMLENIDLRA QLPQIEQPWL
210 220 230 240 250
RVWGRLDGLV PRRVPPLMPN HQAHFTDLLL PKASHAPFLS HRDEFLTGLT

DWLNKF
Length:256
Mass (Da):28,558
Last modified:April 29, 2008 - v1
Checksum:iF493E3EB3974EBB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000961 Genomic DNA. Translation: ACA84457.1.
RefSeqiWP_012322806.1. NC_010506.1.

Genome annotation databases

EnsemblBacteriaiACA84457; ACA84457; Swoo_0156.
KEGGiswd:Swoo_0156.
PATRICi23602507. VBISheWoo126588_0160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000961 Genomic DNA. Translation: ACA84457.1.
RefSeqiWP_012322806.1. NC_010506.1.

3D structure databases

ProteinModelPortaliB1KM44.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi392500.Swoo_0156.

Protein family/group databases

ESTHERishewm-bioh. BioH.
MEROPSiS33.994.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA84457; ACA84457; Swoo_0156.
KEGGiswd:Swoo_0156.
PATRICi23602507. VBISheWoo126588_0160.

Phylogenomic databases

eggNOGiENOG4105D3C. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028062.
KOiK02170.
OMAiALMDEQY.
OrthoDBiPOG091H04D0.

Enzyme and pathway databases

UniPathwayiUPA00078.
BioCyciSWOO392500:GI2C-162-MONOMER.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBIOH_SHEWM
AccessioniPrimary (citable) accession number: B1KM44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: September 7, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.