B1KL12 (RNPH_SHEWM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	Swoo_4568

Organism

Shewanella woodyi (strain ATCC 51908 / MS32) [Complete proteome] [HAMAP]

Taxonomic identifier

392500 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › Shewanella woodyi

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP-Rule MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular_function	tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	Ribonuclease PH HAMAP-Rule MF_00564		PRO_1000129374

Sequences

	Sequence		Length	Mass (Da)
	B1KL12 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: C750AD9E34761E79	FASTA	237	25,743
	10 20 30 40 50 60 MRPNDRTPAQ SRPVTITRQF TAHAEGSVLV EFGETKVLCT ASFTEGVPRF LKGKGQGWVT 70 80 90 100 110 120 AEYGMLPRST HSRMDREAAR GKQSGRTQEI QRLIGRSLRA AVDMKALGEN TIVIDCDVIQ 130 140 150 160 170 180 ADGGTRTAAI TGACVALVDA LNWARGKGIL KNNPLKFLIA AVSVGIYKGE PICDLEYIED 190 200 210 220 230 SEAETDMNVV MTETGKMIEI QGTAEGEPFS HEELLSLLEL AKHGIREIVD VQKAALS

References

[1]

"Complete sequence of Shewanella woodyi ATCC 51908." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Zhao J.-S., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51908 / MS32.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000961 Genomic DNA. Translation: ACA88818.1.
RefSeq	YP_001762913.1. NC_010506.1.
3D structure databases
ProteinModelPortal	B1KL12.
SMR	B1KL12. Positions 2-237.
ModBase	Search...
Protein-protein interaction databases
STRING	392500.Swoo_4568.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA88818; ACA88818; Swoo_4568.
GeneID	6118934.
KEGG	swd:Swoo_4568.
PATRIC	23611863. VBISheWoo126588_4701.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
HOGENOM	HOG000229516.
KO	K00989.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Family and domain databases
HAMAP	MF_00564. RNase_PH.
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_SHEWM

Accession

Primary (citable) accession number: B1KL12

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program