Reviewed, UniProtKB/Swiss-Prot B1KJM4 (KYNU_SHEWM)

Names and origin

Protein names

Recommended name:
    Kynureninase
    EC=3.7.1.3
Alternative name(s):
    L-kynurenine hydrolase

Gene names

Name:	kynU
Ordered Locus Names:	Swoo_1405

Organism

Shewanella woodyi (strain ATCC 51908 / MS32) [Complete proteome] [HAMAP]

Taxonomic identifier

392500 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively By similarity.
Catalytic activity	L-kynurenine + H2O = anthranilate + L-alanine. L-3-hydroxykynurenine + H2O = 3-hydroxyanthranilate + L-alanine.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1. Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the kynureninase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro tryptophan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	kynureninase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Kynureninase		PRO_0000357012
Regions
	Region	133 – 136	4	Pyridoxal phosphate binding By similarity
Sites
	Binding site	105	1	Pyridoxal phosphate; via amide nitrogen By similarity
	Binding site	106	1	Pyridoxal phosphate By similarity
	Binding site	218	1	Pyridoxal phosphate By similarity
	Binding site	221	1	Pyridoxal phosphate By similarity
	Binding site	243	1	Pyridoxal phosphate By similarity
	Binding site	274	1	Pyridoxal phosphate By similarity
	Binding site	302	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	244	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1KJM4-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: BCF6BDB84CF10CDC	FASTA	430	48,545
	10 20 30 40 50 60 MIFENTLAFA QQQDRNDPLA HYSDQFHHPV IDGKEVLYFT GNSLGLQPKT AKEHINQELE 70 80 90 100 110 120 DWAKWGVEGH FHAVNPWVSY HEILTPASAE LVGANESEVV CMNSLTTNLH LLFVSFYKPT 130 140 150 160 170 180 AKRFKIISEA KMFPSDRYLL ETQVRHHGLD PDDAIIEISP REGEYLIREE DIIAAVNDNA 190 200 210 220 230 240 DELALLFFGG VNYFTGQLFD MQRLTKAAHG VGALAGFDLA HAVGNVPMHL HDWDVDFAAW 250 260 270 280 290 300 CTYKYLNSSA GNVGGIFVND RHGNNTKINR FGGWWGHNKE RRFLMENSFE PMTGAEGWQI 310 320 330 340 350 360 SNAPVMGMAI LKSSLDIFHE AGIENLRAKS LKLTAYLEFV FNDIVNQFTD IKLEIITPTD 370 380 390 400 410 420 PTQRGCQLSI KLVGTNKEFF EALTKAGVIA DFREPDVIRL APTPLYNSFE DVYLLGQTLK 430 VLAQNWRQHG

References

[1]

"Complete sequence of Shewanella woodyi ATCC 51908." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Zhao J.-S., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000961 Genomic DNA. Translation: ACA85697.1.
RefSeq	YP_001759792.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6115645.
GenomeReviews	Gene locus Swoo_1405 in contig CP000961_GR.
KEGG	swd:Swoo_1405.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	WQPLSGW.
Family and domain databases
InterPro	IPR010111. Kynureninase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR14084. Kynureninase. 1 hit.
TIGRFAMs	TIGR01814. kynureninase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KYNU_SHEWM

Accession

Primary (citable) accession number: B1KJM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	April 29, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)