B1KJM2 (T23O_SHEWM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	Swoo_1403

Organism

Shewanella woodyi (strain ATCC 51908 / MS32) [Complete proteome] [HAMAP]

Taxonomic identifier

392500 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Tryptophan 2,3-dioxygenase		PRO_0000360134
Regions
	Region	30 – 34	5	Substrate binding By similarity
	Region	55 – 59	5	Substrate binding By similarity
Sites
	Metal binding	244	1	Iron (heme axial ligand) By similarity
	Binding site	117	1	Substrate By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	128	1	Heme By similarity
	Binding site	258	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1KJM2 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: B16B1ADC21670C1E	FASTA	286	33,077
	10 20 30 40 50 60 MVCPHNNPKQ GNTREMEDSI HTDFNNDMSY GDYLCLEQVL SAQHPQSEVH DEMLFIIIHQ 70 80 90 100 110 120 TSELWLKLAG NELDTMIHNV QQGDFSHAFK VISRVKQILN QLTQSWNILS TLTPVDYLKF 130 140 150 160 170 180 RDALGRSSGF QSYGYRKIEF LLGNKNADLI QVHESNEQVH SELQGILERP SLYDEVIRVL 190 200 210 220 230 240 HKQGLPIDDS ALNRDFTQPY QANESVLNAW LSVYRNADEH FELYELAEKL IDIEDAFQQW 250 260 270 280 RFKHMYAVQR IIGNKMGTGG SSGVSFLKKA LDISFFPELF ELRTHL

References

[1]

"Complete sequence of Shewanella woodyi ATCC 51908." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Zhao J.-S., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51908 / MS32.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000961 Genomic DNA. Translation: ACA85695.1.
RefSeq	YP_001759790.1. NC_010506.1.
3D structure databases
ProteinModelPortal	B1KJM2.
SMR	B1KJM2. Positions 26-286.
ModBase	Search...
Protein-protein interaction databases
STRING	B1KJM2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6115643.
GenomeReviews	Gene locus Swoo_1403 in contig CP000961_GR.
KEGG	swd:Swoo_1403.
PATRIC	23605141. VBISheWoo126588_1461.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG647485.
OMA	KLLVDQV.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. Trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_SHEWM

Accession

Primary (citable) accession number: B1KJM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program