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B1KIT5

- SYI_SHEWM

UniProt

B1KIT5 - SYI_SHEWM

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Swoo_1291
Organism
Shewanella woodyi (strain ATCC 51908 / MS32)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylate By similarity
Binding sitei608 – 6081ATP By similarity
Metal bindingi903 – 9031Zinc By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi923 – 9231Zinc By similarity
Metal bindingi926 – 9261Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSWOO392500:GI2C-1309-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Swoo_1291
OrganismiShewanella woodyi (strain ATCC 51908 / MS32)
Taxonomic identifieri392500 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000002168: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Isoleucine--tRNA ligaseUniRule annotationPRO_1000189196Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi392500.Swoo_1291.

Structurei

3D structure databases

ProteinModelPortaliB1KIT5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi605 – 6095"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiEPVMLKS.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1KIT5-1 [UniParc]FASTAAdd to Basket

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MSDYKSTLNL PETEFPMRGN LANREPVMLK SWAEDDLYQQ IRDSRIGRKP    50
FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKTMSGFDAP YIPGWDCHGL 100
PIELKVEQKV GKPGHKVTAA EFRVKCREYA AKQVDGQRDD FMRLGVFGDW 150
HNPYLTMDYS TEANIVRSLS KVIDSGHLHK GVKPVHWCTE CGSALAEAEV 200
EYEDKKSPAI DVAFAAADKV TLLAKFGVEE CSGSVSMVIW TTTPWTLPAN 250
RALSVAGDIE YALVEFVKGD KTSSVILAET LVESCMERYG VDSHNVLGKT 300
SGQSLELLRF NHPFYDFDVP VILGEHVTVD SGTGVVHTAP GHGQDDFVVG 350
QKYGLEVANP VGDNGVYKSD TEIFAGQHVF KANDNVVALL EEKGALIKLE 400
NILHSYPHCW RHKTPIIFRA TPQWFISMEQ KGLRKQALNE IEQTQWIPDW 450
GQSRIEKMVE NRPDWCISRQ RTWGVPIALF VHRETEELHP DSSSLMERVA 500
NKIEQEGIQA WWDLDAAELL GEEADQYRKV TDTLDVWYDS GSSFSSVVAS 550
RPEFQGHDID LYLEGSDQHR GWFMSSLMIS TAMNGKAPYK QVLTHGFTVD 600
GNGRKMSKSV GNVIAPQTVT NKLGADILRL WVAATDYSGE MTVSDEILKR 650
SADAYRRIRN TARFLLANIN GFNPETDLVA VEEMVALDRW AVRRAAALQE 700
ELLEAYEQYN FHVVTQKLMQ FCSVELGSFY LDIIKDRQYT AKGDSHARRS 750
CQSALYLISE AMVRWIAPIL SFTADEVWQL LPGQRDKYVF TQEWFQGLKS 800
VTLESDLSDD FWSELLTVRG EVNKVIEQAR REKQVGGSLE AEITLYADDA 850
LSTALATLGD ELRFVLLTSK TQILDLSAAP ADAIETELSS LKLGLKKAET 900
EKCERCWHHR EDVGQVATHP TLCTRCVTNI EGDGEVRQFA 940
Length:940
Mass (Da):105,573
Last modified:April 29, 2008 - v1
Checksum:i9D57D8E96617ACED
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000961 Genomic DNA. Translation: ACA85583.1.
RefSeqiWP_012323929.1. NC_010506.1.
YP_001759678.1. NC_010506.1.

Genome annotation databases

EnsemblBacteriaiACA85583; ACA85583; Swoo_1291.
GeneIDi6115526.
KEGGiswd:Swoo_1291.
PATRICi23604891. VBISheWoo126588_1341.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000961 Genomic DNA. Translation: ACA85583.1 .
RefSeqi WP_012323929.1. NC_010506.1.
YP_001759678.1. NC_010506.1.

3D structure databases

ProteinModelPortali B1KIT5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 392500.Swoo_1291.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACA85583 ; ACA85583 ; Swoo_1291 .
GeneIDi 6115526.
KEGGi swd:Swoo_1291.
PATRICi 23604891. VBISheWoo126588_1341.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi EPVMLKS.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SWOO392500:GI2C-1309-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51908 / MS32.

Entry informationi

Entry nameiSYI_SHEWM
AccessioniPrimary (citable) accession number: B1KIT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 29, 2008
Last modified: September 3, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi