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B1KIT5

- SYI_SHEWM

UniProt

B1KIT5 - SYI_SHEWM

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Shewanella woodyi (strain ATCC 51908 / MS32)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
    Binding sitei608 – 6081ATPUniRule annotation
    Metal bindingi903 – 9031ZincUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi923 – 9231ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSWOO392500:GI2C-1309-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Swoo_1291
    OrganismiShewanella woodyi (strain ATCC 51908 / MS32)
    Taxonomic identifieri392500 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000002168: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 940940Isoleucine--tRNA ligasePRO_1000189196Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi392500.Swoo_1291.

    Structurei

    3D structure databases

    ProteinModelPortaliB1KIT5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi605 – 6095"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiEPVMLKS.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1KIT5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKSTLNL PETEFPMRGN LANREPVMLK SWAEDDLYQQ IRDSRIGRKP    50
    FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKTMSGFDAP YIPGWDCHGL 100
    PIELKVEQKV GKPGHKVTAA EFRVKCREYA AKQVDGQRDD FMRLGVFGDW 150
    HNPYLTMDYS TEANIVRSLS KVIDSGHLHK GVKPVHWCTE CGSALAEAEV 200
    EYEDKKSPAI DVAFAAADKV TLLAKFGVEE CSGSVSMVIW TTTPWTLPAN 250
    RALSVAGDIE YALVEFVKGD KTSSVILAET LVESCMERYG VDSHNVLGKT 300
    SGQSLELLRF NHPFYDFDVP VILGEHVTVD SGTGVVHTAP GHGQDDFVVG 350
    QKYGLEVANP VGDNGVYKSD TEIFAGQHVF KANDNVVALL EEKGALIKLE 400
    NILHSYPHCW RHKTPIIFRA TPQWFISMEQ KGLRKQALNE IEQTQWIPDW 450
    GQSRIEKMVE NRPDWCISRQ RTWGVPIALF VHRETEELHP DSSSLMERVA 500
    NKIEQEGIQA WWDLDAAELL GEEADQYRKV TDTLDVWYDS GSSFSSVVAS 550
    RPEFQGHDID LYLEGSDQHR GWFMSSLMIS TAMNGKAPYK QVLTHGFTVD 600
    GNGRKMSKSV GNVIAPQTVT NKLGADILRL WVAATDYSGE MTVSDEILKR 650
    SADAYRRIRN TARFLLANIN GFNPETDLVA VEEMVALDRW AVRRAAALQE 700
    ELLEAYEQYN FHVVTQKLMQ FCSVELGSFY LDIIKDRQYT AKGDSHARRS 750
    CQSALYLISE AMVRWIAPIL SFTADEVWQL LPGQRDKYVF TQEWFQGLKS 800
    VTLESDLSDD FWSELLTVRG EVNKVIEQAR REKQVGGSLE AEITLYADDA 850
    LSTALATLGD ELRFVLLTSK TQILDLSAAP ADAIETELSS LKLGLKKAET 900
    EKCERCWHHR EDVGQVATHP TLCTRCVTNI EGDGEVRQFA 940
    Length:940
    Mass (Da):105,573
    Last modified:April 29, 2008 - v1
    Checksum:i9D57D8E96617ACED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000961 Genomic DNA. Translation: ACA85583.1.
    RefSeqiWP_012323929.1. NC_010506.1.
    YP_001759678.1. NC_010506.1.

    Genome annotation databases

    EnsemblBacteriaiACA85583; ACA85583; Swoo_1291.
    GeneIDi6115526.
    KEGGiswd:Swoo_1291.
    PATRICi23604891. VBISheWoo126588_1341.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000961 Genomic DNA. Translation: ACA85583.1 .
    RefSeqi WP_012323929.1. NC_010506.1.
    YP_001759678.1. NC_010506.1.

    3D structure databases

    ProteinModelPortali B1KIT5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 392500.Swoo_1291.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACA85583 ; ACA85583 ; Swoo_1291 .
    GeneIDi 6115526.
    KEGGi swd:Swoo_1291.
    PATRICi 23604891. VBISheWoo126588_1341.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi EPVMLKS.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SWOO392500:GI2C-1309-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51908 / MS32.

    Entry informationi

    Entry nameiSYI_SHEWM
    AccessioniPrimary (citable) accession number: B1KIT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3