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B1KI99 (DDL_SHEWM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Swoo_2674
OrganismShewanella woodyi (strain ATCC 51908 / MS32) [Complete proteome] [HAMAP]
Taxonomic identifier392500 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence ACA86950.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341172

Regions

Domain124 – 329206ATP-grasp
Nucleotide binding154 – 20956ATP By similarity

Sites

Metal binding2831Magnesium or manganese 1 By similarity
Metal binding2961Magnesium or manganese 1 By similarity
Metal binding2961Magnesium or manganese 2 By similarity
Metal binding2981Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B1KI99 [UniParc].

Last modified June 10, 2008. Version 2.
Checksum: 70EA9D910A16C81E

FASTA33537,321
        10         20         30         40         50         60 
MSQINLLLLC GGGGDEHAIS LMSADFLETS LAKEPEINLL RVELDGQGHY RTQDGRSCEL 

        70         80         90        100        110        120 
TNRKEIRFAA EEQAPWPVDY VIPCIHGFPG ETGDIQSYFD LIKLPFFGCD SESSSNCFNK 

       130        140        150        160        170        180 
VTAKMWFSAL GVPNTPYIFL NELNQESIAQ TKQALENWGS IFIKAASQGS SVGCYRVDSQ 

       190        200        210        220        230        240 
DELVSSLEQA FSFSPYVIVE KTINARELEV AAYELNGEIV ATKPGEIICA SNTFYSFDEK 

       250        260        270        280        290        300 
YAANSKAETK VEADVSEEVA QQIQAYAIKV FKGMKLSHLS RIDFFLTEDN EILLNEINTF 

       310        320        330 
PGLTPISMFP KMLQNHGDDF THYLYSNIKS QLVSA 

« Hide

References

[1]"Complete sequence of Shewanella woodyi ATCC 51908."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Lykidis A., Zhao J.-S., Richardson P.
Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51908 / MS32.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000961 Genomic DNA. Translation: ACA86950.1. Different initiation.
RefSeqYP_001761045.1. NC_010506.1.

3D structure databases

ProteinModelPortalB1KI99.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING392500.Swoo_2674.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA86950; ACA86950; Swoo_2674.
GeneID6116973.
KEGGswd:Swoo_2674.
PATRIC23607843. VBISheWoo126588_2753.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAGHILANI.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycSWOO392500:GI2C-2756-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_SHEWM
AccessionPrimary (citable) accession number: B1KI99
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: May 14, 2014
This is version 43 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways