B1KHT1 (TPMT_SHEWM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiopurine S-methyltransferase
EC=2.1.1.67

Alternative name(s):
Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	Swoo_4153

Organism

Shewanella woodyi (strain ATCC 51908 / MS32) [Complete proteome] [HAMAP]

Taxonomic identifier

392500 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity HAMAP MF_00812.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000134081
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	127	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1KHT1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 5038EA48933B2C8F	FASTA	223	25,370
	10 20 30 40 50 60 MEPDFWHSKW NAEQIGFHLN EVNPLLIKYW PALDLAPNSQ VFVPLCGKSL DLCYLAELGH 70 80 90 100 110 120 KVVGCELNQT AVEQFFAENS LPHLMTPAKE SISELNRFDA SDITLYQGDL FSLTPEELSG 130 140 150 160 170 180 VDAFYDRAAL IAWPESMRLA YVEKLIELLP PKSMGLLVTL DYPQEALKGP PFAVSNDWVM 190 200 210 220 ANMADYFELE LLSSEDVLNE NPRFVNKQVP WLTESVYQLK RRG

References

[1]

"Complete sequence of Shewanella woodyi ATCC 51908." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Zhao J.-S., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51908 / MS32.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000961 Genomic DNA. Translation: ACA88409.1.
RefSeq	YP_001762504.1. NC_010506.1.
3D structure databases
ProteinModelPortal	B1KHT1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6118504.
GenomeReviews	Gene locus Swoo_4153 in contig CP000961_GR.
KEGG	swd:Swoo_4153.
PATRIC	23610979. VBISheWoo126588_4274.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG444929.
OMA	FAVSNDW.
ProtClustDB	PRK13255.
Family and domain databases
HAMAP	MF_00812. Thiopur_methtran. [Tree]
InterPro	IPR022474. Thiopur_S-MeTfrase_Se/Te_detox. IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
KO	K00569.
PANTHER	PTHR10259. PTHR10259. 1 hit.
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
TIGRFAMs	TIGR03840. TMPT_Se_Te. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_SHEWM

Accession

Primary (citable) accession number: B1KHT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program