B1KD83 (GLAA_SHEWM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-galactosidase A
EC=3.2.1.n1

Alternative name(s):
Exo-alpha-galactosidase A
EC=3.2.1.22

Gene names

Name:	glaA
Ordered Locus Names:	Swoo_3656

Organism

Shewanella woodyi (strain ATCC 51908 / MS32) [Complete proteome] [HAMAP]

Taxonomic identifier

392500 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Alpha-galactosidase that specifically removes branched alpha-1,3-linked galactose residues present in blood group B antigens. Has no activity toward linear alpha-1,3-linked galactose residues By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Subcellular location	Cell membrane; Lipid-anchor Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 110 family. A subfamily. Contains 5 PbH1 repeats.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Lipoprotein Palmitate
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 608	587	Alpha-1,3-galactosidase A		PRO_5000316297
Regions
	Repeat	262 – 292	31	PbH1 1
	Repeat	318 – 340	23	PbH1 2
	Repeat	426 – 448	23	PbH1 3
	Repeat	449 – 470	22	PbH1 4
	Repeat	481 – 507	27	PbH1 5
Amino acid modifications
	Lipidation	22	1	N-palmitoyl cysteine Potential
	Lipidation	22	1	S-diacylglycerol cysteine Potential

Sequences

	Sequence		Length	Mass (Da)
	B1KD83 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 6691BAA70326DB2A	FASTA	608	68,216
	10 20 30 40 50 60 MQRRTFIKSI SAMMATSTTL GCVSNSLATE KHVSITDFGL RPNSGDDAIP ALQKALAYCK 70 80 90 100 110 120 KHPGTSLIFP LGRYDFWSTK ADRDFYYISN NDDGIKPVAF PLTEFKNLII DGQGSRFVFH 130 140 150 160 170 180 GGMVPVIVRE SSGITLKNFS VDWDVPFHCE GIVEAVNKKE SYVDLKIKDG FSYKVENGRF 190 200 210 220 230 240 IFVGEGFENP VIKNLLEFDT KRQKQAYMAR DNFQREIQPV TTEIRPGVLR LQGKYPTWPK 250 260 270 280 290 300 VGNTLLLMQE RRDWPAFSVQ DTKNTWLENI DIHHSGAMGV IAQLADGIKL DNVNIHPKDG 310 320 330 340 350 360 SGRTVSTTVD ATHFINCKGH VVLKDCLFQG QIDDGTNVHG MYARVAEIHD SNTITFELVH 370 380 390 400 410 420 YQQLGIDIFK PGSKVNFSDS VLNVFHDNVV DKTVRQDAKY WTVSFKEPLD TALKVDDVLD 430 440 450 460 470 480 NMTWQPDHVM ISGCRFTGNR ARGALLTVSG KIIVENNYFS TPMMAIKIGS GGLKWYESGP 490 500 510 520 530 540 VESVEIRNNI FDNCNYAKDS SAIDIVPGRK SKATTPYHQN VKIYQNEFRV YNERVLTTSR 550 560 570 580 590 600 TQGISFVSNK IVKTSEYPER KSAFAPIHVE RSSNFVYQGN DFIGFGHSEL LFIDGQLVSG SGKLEGEG

References

[1]

"Complete sequence of Shewanella woodyi ATCC 51908." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Zhao J.-S., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51908 / MS32.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000961 Genomic DNA. Translation: ACA87918.1.
RefSeq	YP_001762013.1. NC_010506.1.
3D structure databases
ProteinModelPortal	B1KD83.
ModBase	Search...
Protein-protein interaction databases
STRING	B1KD83.
Protein family/group databases
CAZy	GH110. Glycoside Hydrolase Family 110.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6117990.
GenomeReviews	Gene locus Swoo_3656 in contig CP000961_GR.
KEGG	swd:Swoo_3656.
PATRIC	23609933. VBISheWoo126588_3767.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LTWTPEV.
ProtClustDB	CLSK823424.
Family and domain databases
InterPro	IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 3 hits.
SMART	SM00710. PbH1. 5 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLAA_SHEWM

Accession

Primary (citable) accession number: B1KD83

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program