Reviewed, UniProtKB/Swiss-Prot B1KD54 (RLMG_SHEWM)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	Swoo_3625

Organism

Shewanella woodyi (strain ATCC 51908 / MS32) [Complete proteome] [HAMAP]

Taxonomic identifier

392500 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366520

Sequences

	Sequence		Length	Mass (Da)
	B1KD54-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 4FAC427C318A57A7	FASTA	419	46,575
	10 20 30 40 50 60 MTTQFSVAGI ELELARYPKD QESNLQAWDA ADEHLIKHLI ETEQTPVVTA IINDNFGALT 70 80 90 100 110 120 ACLRSIAPTW PLMVETDAKT SLLGNLQNLA TNNLSSEGIE WLNSREALPE QIELVLMKLP 130 140 150 160 170 180 KNLTYFAHQL NRLSQVLPKG TQVLISAKAK SINKSVLELI GKNLGSASAS LTWKKTRVIT 190 200 210 220 230 240 CISDGEIRSL PKEMQWSVPR LNLEIRNLSN VFAANKLDIG AEIMLENMPK GDFKSIIDLG 250 260 270 280 290 300 CGNGILGLHA KQLFPQAYIH FVDDSEMAIE SAKQNWALNK LDTQGLVGEQ ATFGWDDCLT 310 320 330 340 350 360 HMSEGVRPDL VLCNPPFHQG EAITDHIAWQ MFLQSWRALK NGGILHVVGN RHLAYHIKLQ 370 380 390 400 410 RIFKNCTTVA SNGKFVILQA QKISKKAEPF ETHPTEAEAK VEVTESKPHP QSSLYGTKK

References

[1]

"Complete sequence of Shewanella woodyi ATCC 51908." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Zhao J.-S., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000961 Genomic DNA. Translation: ACA87889.1.
RefSeq	YP_001761984.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6117959.
GenomeReviews	Gene locus Swoo_3625 in contig CP000961_GR.
KEGG	swd:Swoo_3625.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG321931.
OMA	CGNGVLG.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_SHEWM

Accession

Primary (citable) accession number: B1KD54

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	January 19, 2010
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)