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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadB

Organism
Shewanella woodyi (strain ATCC 51908 / MS32)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.UniRule annotation

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation
(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei119Important for catalytic activityUniRule annotation1
Sitei139Important for catalytic activityUniRule annotation1
Binding sitei296SubstrateUniRule annotation1
Binding sitei324NAD; via amide nitrogenUniRule annotation1
Binding sitei343NADUniRule annotation1
Binding sitei407NADUniRule annotation1
Binding sitei429NADUniRule annotation1
Active sitei450For 3-hydroxyacyl-CoA dehydrogenase activityUniRule annotation1
Binding sitei453NADUniRule annotation1
Binding sitei500SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi400 – 402NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation, EC:5.3.3.8UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadBUniRule annotation
Ordered Locus Names:Swoo_0021
OrganismiShewanella woodyi (strain ATCC 51908 / MS32)
Taxonomic identifieri392500 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000002168 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001860551 – 716Fatty acid oxidation complex subunit alphaAdd BLAST716

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).UniRule annotation

Protein-protein interaction databases

STRINGi392500.Swoo_0021.

Structurei

3D structure databases

ProteinModelPortaliB1KCZ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 189Enoyl-CoA hydratase/isomeraseUniRule annotationAdd BLAST189
Regioni311 – 7163-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST406

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108EIM. Bacteria.
COG1024. LUCA.
COG1250. LUCA.
HOGENOMiHOG000261344.
KOiK01825.
OMAiYKAKRQP.
OrthoDBiPOG091H00UW.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01621. FadB. 1 hit.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR012799. FadB.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF51735. SSF51735. 1 hit.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02437. FadB. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1KCZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYQSPTIQV ELLEDNIARL CFNAAGSVNK LDRETIDSLN AALDAIKQDT
60 70 80 90 100
NIQGLVLTSG KGAFIVGADI TEFLGLFAQE ESVLLPWIAE ANVVFNKIED
110 120 130 140 150
LPFPTISAIN GFALGGGFET VLATDFRIAD TTAKLGLPET KLGLIPGFGG
160 170 180 190 200
TVRLPRLIGA DNALEWITTG KDHKPEAALK VGAIDAVVAP ENLEASAIAM
210 220 230 240 250
LKEALSEKLD WQARRAKKQA PLNLPKLEAM MSFATAKGMV FKVAGKHYPA
260 270 280 290 300
PMTAISVIEQ AARSERADAL MVEHQAFIKL AKTDVAQALI GIFLNDQLVK
310 320 330 340 350
GKAKKAGKLA KNVDSAAVLG AGIMGGGIAY QSASKGTPIV MKDIAQPALD
360 370 380 390 400
LGLGEASKLL AAQIKRGRST PEKMAKVLNN ITATLDYAPV KDVDVVVEAV
410 420 430 440 450
VEHPKVKSMV LAEVEQNVSD DAIITSNTST ISINLLAKSL KKPERFCGMH
460 470 480 490 500
FFNPVHKMPL VEVIRGENSS EETIASVVAY ASKMGKTPIV VNDCPGFFVN
510 520 530 540 550
RVLFPYFAGF SGLLADGADF AAIDKVMEKQ FGWPMGPAYL LDVVGIDTGH
560 570 580 590 600
HAQAVMAEGF PDRMGKDGKD AIDIMFDSER FGQKNNKGFY AYSVDRRGKP
610 620 630 640 650
KKDLDPTSYE LLGAEFGDLK AFESDEIIAR TMIPMIIETV RCLEEGIIAT
660 670 680 690 700
PAEADMGLVF GLGFPPFRGG VFRYIDTMGV ANFVALADKY AHLGGLYQVT
710
DAMRELAANN GSYYQA
Length:716
Mass (Da):76,934
Last modified:April 29, 2008 - v1
Checksum:i1338DB146E80536A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000961 Genomic DNA. Translation: ACA84322.1.
RefSeqiWP_012322671.1. NC_010506.1.

Genome annotation databases

EnsemblBacteriaiACA84322; ACA84322; Swoo_0021.
KEGGiswd:Swoo_0021.
PATRICi23602221. VBISheWoo126588_0021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000961 Genomic DNA. Translation: ACA84322.1.
RefSeqiWP_012322671.1. NC_010506.1.

3D structure databases

ProteinModelPortaliB1KCZ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi392500.Swoo_0021.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA84322; ACA84322; Swoo_0021.
KEGGiswd:Swoo_0021.
PATRICi23602221. VBISheWoo126588_0021.

Phylogenomic databases

eggNOGiENOG4108EIM. Bacteria.
COG1024. LUCA.
COG1250. LUCA.
HOGENOMiHOG000261344.
KOiK01825.
OMAiYKAKRQP.
OrthoDBiPOG091H00UW.

Enzyme and pathway databases

UniPathwayiUPA00659.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01621. FadB. 1 hit.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR012799. FadB.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF51735. SSF51735. 1 hit.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02437. FadB. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFADB_SHEWM
AccessioniPrimary (citable) accession number: B1KCZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 29, 2008
Last modified: November 2, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.