B1K5S8 (METE_BURCC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	Bcenmc03_4982

Organism

Burkholderia cenocepacia (strain MC0-3) [Complete proteome] [HAMAP]

Taxonomic identifier

406425 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097818
Sites
	Metal binding	650	1	Zinc By similarity
	Metal binding	652	1	Zinc By similarity
	Metal binding	735	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1K5S8 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 5323BF8E96F77AC2	FASTA	764	85,630
	10 20 30 40 50 60 MVTTHNLGFP RIGAKRELKF GLERYWKGES SRDELKALGA ELRRRHWHDQ RDLDLAPIGD 70 80 90 100 110 120 FAFYDQVLDM SFTLGNLPKR VQDFHGDALD NYFRVARGRS AQSAEEHAAC CGGVAAGEMT 130 140 150 160 170 180 KWFDTNYHYI VPEFHADTNF SLDPSRLLQQ LAEANAQGVN AKPVILGPVT YLWLGKAKDD 190 200 210 220 230 240 SDRLALLPKL LPVYGALLDT LTAQGVEWVQ IDEPILVTEL DAEWRQAFRI AYAALETRRI 250 260 270 280 290 300 KLLLATYFGQ LQDNLTLAAS LPVDGLHIDA INARDEVDAL VRELPAERVL SVGAINGRNI 310 320 330 340 350 360 WKTDLNAALD WLEPLAKQLG DRLWLAPSCS LLHVPVDLAS EEKLDAEIRS WLAFALQKLD 370 380 390 400 410 420 ELKVLATALN EGRDKVADAL AANAAAIDSR RRSPRVNNPA VKAAIARIDA RLGNRTSPYT 430 440 450 460 470 480 QRASKQSARL NLPAFPTTTI GSFPQTAEIR QARSRFKAGA LDEAGYRKAM QAEIERSVRE 490 500 510 520 530 540 QESLELDVLV HGEAERNDMV EYFGEQLDGY AFSQFGWVQS YGSRCVKPPI LFGDISRPKA 550 560 570 580 590 600 MTVEWIAYAQ SLTRKPMKGM LTGPVTILNW SFVRDDQPRA VSCYQLALAI REEVLDLEKA 610 620 630 640 650 660 GVRVIQIDEA ALREGLPLRR AQWSEYLKWA VESFRITANG VQDDTQIHTH MCYSEFNDII 670 680 690 700 710 720 ASIADMDADV ITIETSRSDM ELLDAFDTFK YPNEIGPGVY DIHSPNIPTQ DHIVGLMRKA 730 740 750 760 AERIPAERLW VNPDCGLKTR QWAEVIPALT NMVAAAKMLR NQVQ

References

[1]

"Complete sequence of chromosome 2 of Burkholderia cenocepacia MC0-3." Copeland A., Lucas S., Lapidus A., Barry K., Bruce D., Goodwin L., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Tiedje J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC0-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000959 Genomic DNA. Translation: ACA94112.1.
RefSeq	YP_001778602.1. NC_010515.1.
3D structure databases
ProteinModelPortal	B1K5S8.
SMR	B1K5S8. Positions 3-762.
ModBase	Search...
Protein-protein interaction databases
STRING	B1K5S8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6127794.
GenomeReviews	Gene locus Bcenmc03_4982 in contig CP000959_GR.
KEGG	bcm:Bcenmc03_4982.
PATRIC	19099642. VBIBurCen61509_6282.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BURCC

Accession

Primary (citable) accession number: B1K5S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program