B1K0J5 (FMT_BURCC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	Bcenmc03_3142

Organism

Burkholderia cenocepacia (strain MC0-3) [Complete proteome] [HAMAP]

Taxonomic identifier

406425 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex › Burkholderia cenocepacia

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182
Sequence similarities	Belongs to the fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182		PRO_1000098384
Regions
	Region	121 – 124	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1K0J5 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: C12C58BD62CD8345	FASTA	330	34,405
	10 20 30 40 50 60 MTHTLRVIFA GTPEFAAAAL AAIHKAGFPV PLVLTQPDRP AGRGMKLQAS AVKRYAVEHG 70 80 90 100 110 120 MAVAQPPSLR RAGKYPAEAA DAIELLRTTP HDVMVVAAYG LLLPQEVLDI PRAGCINIHA 130 140 150 160 170 180 SLLPRWRGAA PIHRAIEAGD AETGVTLMQM DVGLDTGAMI EEARIAIAPD DTTATLHDRL 190 200 210 220 230 240 AAAGARLIVD ALVRLERDGT LPATPQPADG VTYAEKIGKH EAALDWRKPA DVLARQVRAF 250 260 270 280 290 300 DPFPGGVATL DGAAIKLWAA EPVAAHGTIA TAAPGTIVEA APEGVVVACG SGALRVTQLQ 310 320 330 KPGGKRLPAR EFLAGSPLAA GQRFALPDVD

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia MC0-3." Copeland A., Lucas S., Lapidus A., Barry K., Bruce D., Goodwin L., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Tiedje J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC0-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000958 Genomic DNA. Translation: ACA92300.1.
RefSeq	YP_001766422.1. NC_010508.1.
3D structure databases
ProteinModelPortal	B1K0J5.
ModBase	Search...
Protein-protein interaction databases
STRING	406425.Bcenmc03_3142.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA92300; ACA92300; Bcenmc03_3142.
GeneID	6124803.
KEGG	bcm:Bcenmc03_3142.
PATRIC	19093578. VBIBurCen61509_3268.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
OMA	GITLMQM.
ProtClustDB	PRK00005.
Enzyme and pathway databases
BioCyc	BCEN406425:GHD9-7035-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR001555. GART_AS. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_BURCC

Accession

Primary (citable) accession number: B1K0J5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program