Reviewed, UniProtKB/Swiss-Prot B1JZE0 (BIOD_BURCC)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	Bcenmc03_2948

Organism

Burkholderia cenocepacia (strain MC0-3) [Complete proteome] [HAMAP]

Taxonomic identifier

406425 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP cobalamin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: InterPro dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Dethiobiotin synthetase HAMAP MF_00336		PRO_1000119861
Regions
	Nucleotide binding	11 – 19	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1JZE0-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: DB74C40F53EFAE98	FASTA	239	25,189
	10 20 30 40 50 60 MSAPLSVFVT GTDTEIGKTF VSAAMLHGFA RHGLRAAALK PIAAGAYERD GVWRNEDADQ 70 80 90 100 110 120 LDAAANVALP PELRTPFLLK APAAPHIVAA QEGVTLDLDT IVACHREALT RADVVVVEGV 130 140 150 160 170 180 GGFRVPLNDT QDTADLAVAL GLPVVLVVGV RLGCISHALL TADAIRQRGL TLAGWVANHV 190 200 210 220 230 DPAMSFADEN VATIRDWLAR ERRAPLIGRI AHMTPAAPES AAAMLDIAAL VESLRTARH

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia MC0-3." Copeland A., Lucas S., Lapidus A., Barry K., Bruce D., Goodwin L., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Tiedje J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000958 Genomic DNA. Translation: ACA92106.1.
RefSeq	YP_001766228.1.
3D structure databases
SMR	B1JZE0. Positions 7-213.
ModBase	Search...
Genome annotation databases
GeneID	6124609.
GenomeReviews	Gene locus Bcenmc03_2948 in contig CP000958_GR.
KEGG	bcm:Bcenmc03_2948.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	ANCVDPN.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BURCC

Accession

Primary (citable) accession number: B1JZE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)