B1JYK1 (QUEF_BURCC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	Bcenmc03_2764

Organism

Burkholderia cenocepacia (strain MC0-3) [Complete proteome] [HAMAP]

Taxonomic identifier

406425 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm By similarity HAMAP MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_1000193214

Sequences

	Sequence		Length	Mass (Da)
	B1JYK1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 4EC7540E5C818645	FASTA	276	30,594
	10 20 30 40 50 60 MNPEHSPLGK ATVYAAQYDA SLLFPIPRAG AREQLGITSA LPFFGTDIWN AYELSWLNTR 70 80 90 100 110 120 GKPQVAIATF YVPAESPNIV ESKSFKLYLG SFAQSKFDSV DAVRDVLKRD VSAACGASVS 130 140 150 160 170 180 VQLVSAHDFA KLEMDELDGL SLDRLDLDTD VYEPDPSLLS AADGENEAPV EETLVSDLLR 190 200 210 220 230 240 SNCPVTGQPD WGSVQIHYVG PQIDHAGLLR YIISFRNHTG FHEQCVERIF LDIMHACKPV 250 260 270 KLAVYARYTR RGGLDINPFR TNYNQPMPDN ARTARQ

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia MC0-3." Copeland A., Lucas S., Lapidus A., Barry K., Bruce D., Goodwin L., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Tiedje J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC0-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000958 Genomic DNA. Translation: ACA91924.1.
RefSeq	YP_001766046.1. NC_010508.1.
3D structure databases
ProteinModelPortal	B1JYK1.
SMR	B1JYK1. Positions 14-276.
ModBase	Search...
Protein-protein interaction databases
STRING	B1JYK1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6124427.
GenomeReviews	Gene locus Bcenmc03_2764 in contig CP000958_GR.
KEGG	bcm:Bcenmc03_2764.
PATRIC	19092768. VBIBurCen61509_2866.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289139.
OMA	CVERIFC.
ProtClustDB	PRK11792.
Family and domain databases
HAMAP	MF_00817. QueF_type2. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K06879.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_BURCC

Accession

Primary (citable) accession number: B1JYK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program