Reviewed, UniProtKB/Swiss-Prot B1JXI9 (T23O_BURCC)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:	kynA
Ordered Locus Names:	Bcenmc03_2603

Organism

Burkholderia cenocepacia (strain MC0-3) [Complete proteome] [HAMAP]

Taxonomic identifier

406425 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Tryptophan 2,3-dioxygenase		PRO_0000360098
Regions
	Region	55 – 59	5	Substrate binding By similarity
	Region	80 – 84	5	Substrate binding By similarity
Sites
	Metal binding	269	1	Iron (heme axial ligand) By similarity
	Binding site	142	1	Substrate By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	153	1	Heme By similarity
	Binding site	283	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1JXI9-1 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: DF821C94A7640746	FASTA	311	35,429
	10 20 30 40 50 60 MQPPGGDAPA GCPFSGARAA QPAQAAHEAP HVPGEADAQA GWHNAQLDFS KSMSYGDYLS 70 80 90 100 110 120 LNSILDAQHP LSPDHNEMLF IIQHQTSELW MKLALFELRG ALDAVRTDAL PPAFKMLARV 130 140 150 160 170 180 SRILEQLVQA WNVLSTMTPS EYSAMRPYLG QSSGFQSYQY RQLEFLLGNK NAQMLQPHAH 190 200 210 220 230 240 RPDILEQVRA TLEAPSFYDE VVRLLARRGF PIAAERLERD WTQPTRHDET VEAAWLEVYR 250 260 270 280 290 300 HPQQHWELYE MAEELVDLED AFRQWRFRHV TTVERIIGFK QGTGGTSGAP YLRKMLDVVL 310 FPELWHVRTT L

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia MC0-3." Copeland A., Lucas S., Lapidus A., Barry K., Bruce D., Goodwin L., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Tiedje J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000958 Genomic DNA. Translation: ACA91764.1. Different initiation.
RefSeq	YP_001765886.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6124267.
GenomeReviews	Gene locus Bcenmc03_2603 in contig CP000958_GR.
KEGG	bcm:Bcenmc03_2603.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_BURCC

Accession

Primary (citable) accession number: B1JXI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 20, 2009
Last modified:	October 13, 2009
This is version 14 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)