Reviewed, UniProtKB/Swiss-Prot B1JW14 (HEMH_BURCC)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	Bcenmc03_0716

Organism

Burkholderia cenocepacia (strain MC0-3) [Complete proteome] [HAMAP]

Taxonomic identifier

406425 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX By similarity.
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Ferrochelatase HAMAP MF_00323		PRO_1000116033
Sites
	Metal binding	214	1	Iron By similarity
	Metal binding	295	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1JW14-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: CFEA7BB5A13CBA72	FASTA	354	39,359
	10 20 30 40 50 60 MRFDLEPPSS VAAAHRIGVL LINLGTPDAP TPRAVRRYLA EFLSDPRVVE IPQAVWQVLL 70 80 90 100 110 120 RTLILPLRGR ASAKKYAAVW MPEGSPLRVY TERQTDSVRH LLTSNGYHVM VDYAMRYGSP 130 140 150 160 170 180 NISHALTQFK RAGVERVLLM PMYPQYSAST TATAFDAAFD ALARMRNQPE VRTVRHYADH 190 200 210 220 230 240 PAYIHALAEQ VRQYWAQHGR PDFAAGDKLV LSFHGVPKRT LDLGDPYHDQ CQQTGALLMA 250 260 270 280 290 300 ALGLSTTECR VTFQSRFGKA EWLQPYTAPT LREFGEAGVR RADVFCPGFT ADCLETIEEI 310 320 330 340 350 GMEVRDEFLA GGGKTFHRIP CLNGASAWIG ALGEIVAENL QGWPVKAAQP EPVN

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cenocepacia MC0-3." Copeland A., Lucas S., Lapidus A., Barry K., Bruce D., Goodwin L., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Tiedje J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000958 Genomic DNA. Translation: ACA89894.1.
RefSeq	YP_001764016.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6122397.
GenomeReviews	Gene locus Bcenmc03_0716 in contig CP000958_GR.
KEGG	bcm:Bcenmc03_0716.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TIEEIGM.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BURCC

Accession

Primary (citable) accession number: B1JW14

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)