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UniProtKB - B1JUF2 (GLND_BURCC)

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Burkholderia cenocepacia (strain MC0-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionHydrolase, Nucleotidyltransferase, Transferase
LigandMagnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:Bcenmc03_2041
OrganismiBurkholderia cenocepacia (strain MC0-3)
Taxonomic identifieri406425 [NCBI]
Taxonomic lineageiBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex ›
Proteomesi
  • UP000002169 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001147521 – 858Bifunctional uridylyltransferase/uridylyl-removing enzymeAdd BLAST858

Structurei

3D structure databases

ProteinModelPortaliB1JUF2.
SMRiB1JUF2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini443 – 565HDPROSITE-ProRule annotationAdd BLAST123
Domaini682 – 763ACT 1UniRule annotationAdd BLAST82
Domaini790 – 858ACT 2UniRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 324UridylyltransferaseAdd BLAST324
Regioni325 – 681Uridylyl-removingAdd BLAST357

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000261778.
KOiK00990.
OMAiARICGYF.
OrthoDBiPOG091H04HO.

Family and domain databases

HAMAPiMF_00277. PII_uridylyl_transf. 1 hit.
InterProiView protein in InterPro
IPR002912. ACT_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR002934. Polymerase_NTP_transf_dom.
IPR010043. UTase/UR.
PANTHERiPTHR13734:SF52. PTHR13734:SF52. 1 hit.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiView protein in SMART
SM00471. HDc. 1 hit.
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiView protein in PROSITE
PS51671. ACT. 2 hits.
PS51831. HD. 1 hit.

Sequencei

Sequence statusi: Complete.

B1JUF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAHAAPSPE ALSRRAEFKA AKTEMLERFR HAANVASLMH ALSKLTDDAL 
        60         70         80         90        100
KRVWDECGLP ATLALVAVGG YGRGELAPYS DVDIVVLLPD AHDAALDARI 
       110        120        130        140        150
ERFIGMAWDL GLEIGSSVRT VAQCIEEASQ DVTVQTSLLE ARRIVGSTAL 
       160        170        180        190        200
FERFTVRYHE ALDARAFFTA KVLEMRQRHA KFQDTPYSLE PNVKESPGGL 
       210        220        230        240        250
RDLQTILWIA RAAGFGSSWR ELDTRGLITD REARELRRNE GFLKTLRARL 
       260        270        280        290        300
HVIAGRRQDM LVFDLQTQAA ESFGYQPTQA KRASEQLMRR YYWAAKAVTQ 
       310        320        330        340        350
LATILIQNIE AQLFPATSGI TRVLSADRFV EKQGMLEIVD DGVFERHPDA 
       360        370        380        390        400
ILEAFLLYET TRGVKGLSAR TLRALYNSRE IMNNAWRRDP QNRATFMRIL 
       410        420        430        440        450
QQPEGITHAF RLMNQTSVLG RYLLNFRRIV GQMQHDLYHV YTVDQHILMV 
       460        470        480        490        500
LRNIRRFAVA EHAHEYPFCS QLIGNFERPW ALYVAALFHD IAKGRGGDHS 
       510        520        530        540        550
TLGMADARRF CREHGIAGDD AALIVWLVQH HLTMSQVAQK QDTSDPEVIK 
       560        570        580        590        600
RFAEVVGNER YLTALYLLTV ADIRGTSPKV WNTWKGKLLE DLYRITLAVL 
       610        620        630        640        650
GGANPDAHSE LKSRQEQALA LLRLETVPDD AHRALWDQLD VGFFLRHDAA 
       660        670        680        690        700
DIAWQTRVLY RHVNAETAIV RARPSPIGDA LQVLVYVKDR PDLFAGICAY 
       710        720        730        740        750
FDRNGLSVLD ARVSTTRHGY ALDNFIVTQT ERDVRYRDIA NLVEQQLATR 
       760        770        780        790        800
LAETASLPEP SKGRLSRLSR TFPITPRVDL RADERGQYYI LSVSANDRPG 
       810        820        830        840        850
LLYSIARVLA EHRIGVHAAR INTLGERVED IFLLAGAGLS DNRLQIQLET 

ELLRAIAV
Length:858
Mass (Da):96,854
Last modified:April 29, 2008 - v1
Checksum:iE85B86E5A00DF810
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000958 Genomic DNA. Translation: ACA91202.1.
RefSeqiWP_012328761.1. NC_010508.1.

Genome annotation databases

EnsemblBacteriaiACA91202; ACA91202; Bcenmc03_2041.
KEGGibcm:Bcenmc03_2041.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000958 Genomic DNA. Translation: ACA91202.1.
RefSeqiWP_012328761.1. NC_010508.1.

3D structure databases

ProteinModelPortaliB1JUF2.
SMRiB1JUF2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA91202; ACA91202; Bcenmc03_2041.
KEGGibcm:Bcenmc03_2041.

Phylogenomic databases

HOGENOMiHOG000261778.
KOiK00990.
OMAiARICGYF.
OrthoDBiPOG091H04HO.

Family and domain databases

HAMAPiMF_00277. PII_uridylyl_transf. 1 hit.
InterProiView protein in InterPro
IPR002912. ACT_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR002934. Polymerase_NTP_transf_dom.
IPR010043. UTase/UR.
PANTHERiPTHR13734:SF52. PTHR13734:SF52. 1 hit.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiView protein in SMART
SM00471. HDc. 1 hit.
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiView protein in PROSITE
PS51671. ACT. 2 hits.
PS51831. HD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLND_BURCC
AccessioniPrimary (citable) accession number: B1JUF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: June 7, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.