UniProtKB - B1JUF2 (GLND_BURCC)
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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD
Organism
Burkholderia cenocepacia (strain MC0-3)
Status
Functioni
Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation
Catalytic activityi
UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation
Cofactori
Mg2+UniRule annotation
Enzyme regulationi
Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation
GO - Molecular functioni
- [protein-PII] uridylyltransferase activity Source: UniProtKB-EC
- hydrolase activity Source: UniProtKB-KW
GO - Biological processi
- nitrogen compound metabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase, Multifunctional enzyme, Nucleotidyltransferase, Transferase |
Ligand | Magnesium |
Enzyme and pathway databases
BioCyci | BCEN406425:G1GBC-2164-MONOMER. |
Names & Taxonomyi
Protein namesi | Recommended name: Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationShort name: UTase/URUniRule annotation Alternative name(s): Bifunctional [protein-PII] modification enzymeUniRule annotation Bifunctional nitrogen sensor proteinUniRule annotation Including the following 2 domains: |
Gene namesi | Name:glnDUniRule annotation Ordered Locus Names:Bcenmc03_2041 |
Organismi | Burkholderia cenocepacia (strain MC0-3) |
Taxonomic identifieri | 406425 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000114752 | 1 – 858 | Bifunctional uridylyltransferase/uridylyl-removing enzymeAdd BLAST | 858 |
Structurei
3D structure databases
ProteinModelPortali | B1JUF2. |
SMRi | B1JUF2. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 443 – 565 | HDPROSITE-ProRule annotationAdd BLAST | 123 | |
Domaini | 682 – 763 | ACT 1UniRule annotationAdd BLAST | 82 | |
Domaini | 790 – 858 | ACT 2UniRule annotationAdd BLAST | 69 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 324 | UridylyltransferaseAdd BLAST | 324 | |
Regioni | 325 – 681 | Uridylyl-removingAdd BLAST | 357 |
Domaini
Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation
Sequence similaritiesi
Belongs to the GlnD family.UniRule annotation
Keywords - Domaini
RepeatPhylogenomic databases
HOGENOMi | HOG000261778. |
KOi | K00990. |
OMAi | ARICGYF. |
OrthoDBi | POG091H04HO. |
Family and domain databases
CDDi | cd00077. HDc. 1 hit. |
HAMAPi | MF_00277. PII_uridylyl_transf. 1 hit. |
InterProi | View protein in InterPro IPR002912. ACT_dom. IPR003607. HD/PDEase_dom. IPR006674. HD_domain. IPR013546. PII_UdlTrfase/GS_AdlTrfase. IPR002934. Polymerase_NTP_transf_dom. IPR010043. UTase/UR. |
PANTHERi | PTHR13734:SF45. PTHR13734:SF45. 2 hits. |
Pfami | View protein in Pfam PF08335. GlnD_UR_UTase. 1 hit. PF01966. HD. 1 hit. PF01909. NTP_transf_2. 1 hit. |
PIRSFi | PIRSF006288. PII_uridyltransf. 1 hit. |
SMARTi | View protein in SMART SM00471. HDc. 1 hit. |
TIGRFAMsi | TIGR01693. UTase_glnD. 1 hit. |
PROSITEi | View protein in PROSITE PS51671. ACT. 2 hits. PS51831. HD. 1 hit. |
i Sequence
Sequence statusi: Complete.
B1JUF2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAHAAPSPE ALSRRAEFKA AKTEMLERFR HAANVASLMH ALSKLTDDAL
60 70 80 90 100
KRVWDECGLP ATLALVAVGG YGRGELAPYS DVDIVVLLPD AHDAALDARI
110 120 130 140 150
ERFIGMAWDL GLEIGSSVRT VAQCIEEASQ DVTVQTSLLE ARRIVGSTAL
160 170 180 190 200
FERFTVRYHE ALDARAFFTA KVLEMRQRHA KFQDTPYSLE PNVKESPGGL
210 220 230 240 250
RDLQTILWIA RAAGFGSSWR ELDTRGLITD REARELRRNE GFLKTLRARL
260 270 280 290 300
HVIAGRRQDM LVFDLQTQAA ESFGYQPTQA KRASEQLMRR YYWAAKAVTQ
310 320 330 340 350
LATILIQNIE AQLFPATSGI TRVLSADRFV EKQGMLEIVD DGVFERHPDA
360 370 380 390 400
ILEAFLLYET TRGVKGLSAR TLRALYNSRE IMNNAWRRDP QNRATFMRIL
410 420 430 440 450
QQPEGITHAF RLMNQTSVLG RYLLNFRRIV GQMQHDLYHV YTVDQHILMV
460 470 480 490 500
LRNIRRFAVA EHAHEYPFCS QLIGNFERPW ALYVAALFHD IAKGRGGDHS
510 520 530 540 550
TLGMADARRF CREHGIAGDD AALIVWLVQH HLTMSQVAQK QDTSDPEVIK
560 570 580 590 600
RFAEVVGNER YLTALYLLTV ADIRGTSPKV WNTWKGKLLE DLYRITLAVL
610 620 630 640 650
GGANPDAHSE LKSRQEQALA LLRLETVPDD AHRALWDQLD VGFFLRHDAA
660 670 680 690 700
DIAWQTRVLY RHVNAETAIV RARPSPIGDA LQVLVYVKDR PDLFAGICAY
710 720 730 740 750
FDRNGLSVLD ARVSTTRHGY ALDNFIVTQT ERDVRYRDIA NLVEQQLATR
760 770 780 790 800
LAETASLPEP SKGRLSRLSR TFPITPRVDL RADERGQYYI LSVSANDRPG
810 820 830 840 850
LLYSIARVLA EHRIGVHAAR INTLGERVED IFLLAGAGLS DNRLQIQLET
ELLRAIAV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000958 Genomic DNA. Translation: ACA91202.1. |
RefSeqi | WP_012328761.1. NC_010508.1. |
Genome annotation databases
EnsemblBacteriai | ACA91202; ACA91202; Bcenmc03_2041. |
KEGGi | bcm:Bcenmc03_2041. |
Similar proteinsi
Entry informationi
Entry namei | GLND_BURCC | |
Accessioni | B1JUF2Primary (citable) accession number: B1JUF2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 24, 2009 |
Last sequence update: | April 29, 2008 | |
Last modified: | February 28, 2018 | |
This is version 66 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |