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Protein

ATP-dependent Clp protease proteolytic subunit

Gene

clpP

Organism
Burkholderia cenocepacia (strain MC0-3)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.UniRule annotation

Catalytic activityi

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).UniRule annotation
Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu-and -Tyr-|-Trp bonds also occurs).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei121 – 1211NucleophileUniRule annotation
Active sitei146 – 1461UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine proteaseUniRule annotation

Enzyme and pathway databases

BioCyciBCEN406425:GHD9-1996-MONOMER.

Protein family/group databases

MEROPSiS14.001.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease proteolytic subunitUniRule annotation (EC:3.4.21.92UniRule annotation)
Alternative name(s):
Endopeptidase ClpUniRule annotation
Gene namesi
Name:clpPUniRule annotation
Ordered Locus Names:Bcenmc03_1946Imported
OrganismiBurkholderia cenocepacia (strain MC0-3)Imported
Taxonomic identifieri406425 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000002169 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB1JTV0.
SMRiB1JTV0. Positions 25-216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S14 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0740.
HOGENOMiHOG000285833.
KOiK01358.
OMAiTAGAKGH.
OrthoDBiEOG6Z3KQ0.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
HAMAPiMF_00444. ClpP.
InterProiIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR018215. ClpP_AS.
[Graphical view]
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiPF00574. CLP_protease. 1 hit.
[Graphical view]
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR00493. clpP. 1 hit.
PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1JTV0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITRAELLDM LASNAPQGFE AQALGLVPIV VETSGRGERS YDIYSRLLKE
60 70 80 90 100
RLVFMVGEVN DQTANLVVAQ LLFLESENPD KDISLYINSP GGSVSAGMAI
110 120 130 140 150
YDTMQFIKPD VSTLCMGLAA SMGAFLLASG AKGKRFALPN SRVMIHQPLG
160 170 180 190 200
GARGQASDIE IQAREILYLK ERLNNLLAQH TGQDVERIAR DTDRDNFMSS
210
EDAKAYGLID QVLLKRP
Length:217
Mass (Da):23,750
Last modified:April 29, 2008 - v1
Checksum:i924F3B6062C85117
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000958 Genomic DNA. Translation: ACA91107.1.
RefSeqiWP_006496351.1. NC_010508.1.

Genome annotation databases

EnsemblBacteriaiACA91107; ACA91107; Bcenmc03_1946.
GeneIDi6933441.
KEGGibcm:Bcenmc03_1946.
PATRICi19091005. VBIBurCen61509_2008.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000958 Genomic DNA. Translation: ACA91107.1.
RefSeqiWP_006496351.1. NC_010508.1.

3D structure databases

ProteinModelPortaliB1JTV0.
SMRiB1JTV0. Positions 25-216.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS14.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA91107; ACA91107; Bcenmc03_1946.
GeneIDi6933441.
KEGGibcm:Bcenmc03_1946.
PATRICi19091005. VBIBurCen61509_2008.

Phylogenomic databases

eggNOGiCOG0740.
HOGENOMiHOG000285833.
KOiK01358.
OMAiTAGAKGH.
OrthoDBiEOG6Z3KQ0.

Enzyme and pathway databases

BioCyciBCEN406425:GHD9-1996-MONOMER.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
HAMAPiMF_00444. ClpP.
InterProiIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR018215. ClpP_AS.
[Graphical view]
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiPF00574. CLP_protease. 1 hit.
[Graphical view]
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR00493. clpP. 1 hit.
PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Burkholderia cenocepacia MC0-3."
    Copeland A., Lucas S., Lapidus A., Barry K., Bruce D., Goodwin L., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Tiedje J., Richardson P.
    Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MC0-3Imported.

Entry informationi

Entry nameiB1JTV0_BURCC
AccessioniPrimary (citable) accession number: B1JTV0
Entry historyi
Integrated into UniProtKB/TrEMBL: April 29, 2008
Last sequence update: April 29, 2008
Last modified: July 22, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.