B1JST8 (GAL1_YERPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactokinase
EC=2.7.1.6

Alternative name(s):
Galactose kinase

Gene names

Name:	galK
Ordered Locus Names:	YPK_2945

Organism

Yersinia pseudotuberculosis serotype O:3 (strain YPIII) [Complete proteome] [HAMAP]

Taxonomic identifier

502800 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia pseudotuberculosis

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P) By similarity. HAMAP-Rule MF_00246
Catalytic activity	ATP + D-galactose = ADP + alpha-D-galactose 1-phosphate. HAMAP-Rule MF_00246
Pathway	Carbohydrate metabolism; galactose metabolism. HAMAP-Rule MF_00246
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00246.
Sequence similarities	Belongs to the GHMP kinase family. GalK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Galactose metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	galactose metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP galactokinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	Galactokinase HAMAP-Rule MF_00246		PRO_1000100851
Regions
	Nucleotide binding	124 – 130	7	ATP By similarity
	Region	34 – 37	4	Substrate binding By similarity
Sites
	Active site	174	1	Proton acceptor By similarity
	Metal binding	130	1	Magnesium By similarity
	Metal binding	162	1	Magnesium By similarity
	Binding site	223	1	Substrate By similarity
	Site	28	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1JST8 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 548D19EB39D13281	FASTA	383	41,866
	10 20 30 40 50 60 MSLKQHTQTI FRQQFDRESD ITIKAPGRVN LIGEHTDYND GFVLPCAINY ETVISCGKRD 70 80 90 100 110 120 DRQIRVIAAD YENQQDIFSL DAPIVPHPEY RWADYVRGVV KHLQMRNADF GGADLVICGN 130 140 150 160 170 180 VPQGAGLSSS ASLEVAVGQA LQSLYQLPLS GVELALNGQE AENQFVGCNC GIMDQLISAL 190 200 210 220 230 240 GKKDHALLID CRTLETRAVP MPENMAVVII NSNIQRGLVD SEYNTRRQQC EAAARFFGVK 250 260 270 280 290 300 ALRDVEPSLF FSIQDELDPV VAKRARHVIS ENARTLAAAD ALAAGNLKLM GQLMQESHIS 310 320 330 340 350 360 MRDDFEITVP PIDRLVEIVK SVIGDQGGVR MTGGGFGGCI IALMPLELVE QVRTTVAQEY 370 380 PAHSGGKKET FYVCQASQGA GLC

References

[1]

"Complete sequence of Yersinia pseudotuberculosis YPIII." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L. , Challacombe J.F., Green L., Lindler L.E., Nikolich M.P., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YPIII.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000950 Genomic DNA. Translation: ACA69219.1.
RefSeq	YP_001721672.1. NC_010465.1.
3D structure databases
ProteinModelPortal	B1JST8.
ModBase	Search...
Protein-protein interaction databases
STRING	502800.YPK_2945.
Proteomic databases
PRIDE	B1JST8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA69219; ACA69219; YPK_2945.
GeneID	6088070.
KEGG	ypy:YPK_2945.
PATRIC	18663437. VBIYerPse127545_3193.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0153.
HOGENOM	HOG000241100.
KO	K00849.
OMA	MVESHHS.
ProtClustDB	PRK05101.
Enzyme and pathway databases
UniPathway	UPA00214.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00246. Galactokinase.
InterPro	IPR000705. Galactokinase. IPR022963. Galactokinase_bac. IPR019741. Galactokinase_CS. IPR019539. GalKase_gal-bd. IPR013750. GHMP_kinase_C_dom. IPR006204. GHMP_kinase_N_dom. IPR006203. GHMP_knse_ATP-bd_CS. IPR006206. Mevalonate/galactokinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Pfam	PF10509. GalKase_gal_bdg. 1 hit. PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000530. Galactokinase. 1 hit.
PRINTS	PR00473. GALCTOKINASE. PR00959. MEVGALKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00131. gal_kin. 1 hit.
PROSITE	PS00106. GALACTOKINASE. 1 hit. PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAL1_YERPY

Accession

Primary (citable) accession number: B1JST8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program