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B1JQU4

- PFKA_YERPY

UniProt

B1JQU4 - PFKA_YERPY

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Yersinia pseudotuberculosis serotype O:3 (strain YPIII)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121ATP; via amide nitrogenUniRule annotation
    Metal bindingi104 – 1041Magnesium; catalyticUniRule annotation
    Active sitei129 – 1291Proton acceptorUniRule annotation
    Binding sitei156 – 1561Allosteric activator ADPUniRule annotation
    Binding sitei164 – 1641Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei213 – 2131Allosteric activator ADPUniRule annotation
    Binding sitei224 – 2241SubstrateUniRule annotation
    Binding sitei245 – 2451Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi73 – 742ATPUniRule annotation
    Nucleotide bindingi103 – 1064ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYPSE502800:GH0W-4188-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:YPK_4126
    OrganismiYersinia pseudotuberculosis serotype O:3 (strain YPIII)
    Taxonomic identifieri502800 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000000721: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327ATP-dependent 6-phosphofructokinasePRO_1000120070Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi502800.YPK_4126.

    Structurei

    3D structure databases

    ProteinModelPortaliB1JQU4.
    SMRiB1JQU4. Positions 1-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni22 – 265Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni55 – 606Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni127 – 1293Substrate bindingUniRule annotation
    Regioni171 – 1733Substrate bindingUniRule annotation
    Regioni187 – 1893Allosteric activator ADP bindingUniRule annotation
    Regioni215 – 2173Allosteric activator ADP bindingUniRule annotation
    Regioni251 – 2544Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000248870.
    KOiK00850.
    OMAiGFGGRCV.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase_I_B1.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1JQU4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKKIGVLTS GGDAPGMNAA IRGVVRAALS AGLDVFGIED GYLGLYENRM    50
    KKLDRYSVSD MINRGGTFLG SARFPEFRDP EVRKVALKNM HERGIDGLVV 100
    IGGDGSYAGA DLLTKEGGIH CVGLPGTIDN DVAGTDYTIG FFTALETVVE 150
    AIDRLRDTSS SHQRISIVEV MGRYCGDLTL AAAIAGGCEF IAIPEVEFKR 200
    DDLVAEIKAG IAKGKKHAIV AITEKLDDID SLAKYIEKET GRETRGTVLG 250
    HIQRGGAPVA YDRILASRMG SYAVDLLLQD HDYKKGGFCV GVQNEKMVHE 300
    LISVCIAPEN KKSKFKEDWY DTAKKLF 327
    Length:327
    Mass (Da):35,411
    Last modified:April 29, 2008 - v1
    Checksum:i0F46B3A8AF0E430D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000950 Genomic DNA. Translation: ACA70385.1.
    RefSeqiYP_001722838.1. NC_010465.1.

    Genome annotation databases

    EnsemblBacteriaiACA70385; ACA70385; YPK_4126.
    GeneIDi6091101.
    KEGGiypy:YPK_4126.
    PATRICi18666059. VBIYerPse127545_4459.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000950 Genomic DNA. Translation: ACA70385.1 .
    RefSeqi YP_001722838.1. NC_010465.1.

    3D structure databases

    ProteinModelPortali B1JQU4.
    SMRi B1JQU4. Positions 1-327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 502800.YPK_4126.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACA70385 ; ACA70385 ; YPK_4126 .
    GeneIDi 6091101.
    KEGGi ypy:YPK_4126.
    PATRICi 18666059. VBIYerPse127545_4459.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000248870.
    KOi K00850.
    OMAi GFGGRCV.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci YPSE502800:GH0W-4188-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase_I_B1.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: YPIII.

    Entry informationi

    Entry nameiPFKA_YERPY
    AccessioniPrimary (citable) accession number: B1JQU4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3