B1JJH6 (RSMB_YERPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase B
EC=2.1.1.176

Alternative name(s):
16S rRNA m5C967 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RsmB

Gene names

Name:	rsmB
Synonyms:	sun
Ordered Locus Names:	YPK_0314

Organism

Yersinia pseudotuberculosis serotype O:3 (strain YPIII) [Complete proteome] [HAMAP]

Taxonomic identifier

502800 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia pseudotuberculosis

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA By similarity. HAMAP-Rule MF_01856
Catalytic activity	S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA. HAMAP-Rule MF_01856
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01856.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmB/NOP family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA (cytosine) methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Ribosomal RNA small subunit methyltransferase B HAMAP-Rule MF_01856		PRO_0000366190
Regions
	Region	254 – 260	7	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	375	1	Nucleophile Potential
	Binding site	277	1	S-adenosyl-L-methionine By similarity
	Binding site	303	1	S-adenosyl-L-methionine By similarity
	Binding site	322	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1JJH6 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 0E18231A62D8782E	FASTA	429	48,444
	10 20 30 40 50 60 MKNTYNLRSI AAKAISQVLD QGQSLSAVLP ELQKNISDKD RALLQELCFG TLRVLPQLEW 70 80 90 100 110 120 CIQQLMARPM TGKQRVFHYL IMVGLYQLIY TRIPPHAALA ETVEGATVLK RPQLKGLING 130 140 150 160 170 180 VLRQFQRQQV ELLERAVNND SHYLHPSWLL ARIKQAYPAQ WQQILDANNQ RPPMWLRVNR 190 200 210 220 230 240 LHHSRSEYLE LLTQADINAE PHPIYRDAVR LITPCAVNHL PGFELGWVTV QDASAQGCVD 250 260 270 280 290 300 LLDPQNGEQI LDLCAAPGGK TTHILEAAPK AHVLAVDIDE QRLSRVKENL QRLQLQAVVR 310 320 330 340 350 360 VGDGRAPDTW CGDQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDISELA QLQSEIIEAI 370 380 390 400 410 420 WPKLKHGGVL VYATCSILPE ENQQQIAAFL QRHPEAQLTE TGTTAAPGKQ NLPHPEDGDG FFYAKIIKK

References

[1]

"Complete sequence of Yersinia pseudotuberculosis YPIII." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L. , Challacombe J.F., Green L., Lindler L.E., Nikolich M.P., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YPIII.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000950 Genomic DNA. Translation: ACA66627.1.
RefSeq	YP_001719080.1. NC_010465.1.
3D structure databases
ProteinModelPortal	B1JJH6.
SMR	B1JJH6. Positions 6-428.
ModBase	Search...
Protein-protein interaction databases
STRING	502800.YPK_0314.
Proteomic databases
PRIDE	B1JJH6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA66627; ACA66627; YPK_0314.
GeneID	6089945.
KEGG	ypy:YPK_0314.
PATRIC	18657626. VBIYerPse127545_0333.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0144.
HOGENOM	HOG000037300.
KO	K03500.
OMA	KPPMWLR.
ProtClustDB	PRK10901.
Enzyme and pathway databases
BioCyc	YPSE502800:GH0W-315-MONOMER.
Family and domain databases
Gene3D	1.10.940.10. 1 hit.
HAMAP	MF_01856. 16SrRNA_methyltr_B.
InterPro	IPR001678. Fmu/NOL1/Nop2p. IPR018314. Fmu/NOL1/Nop2p_CS. IPR006027. NusB_RsmB_TIM44. IPR023267. RCMT. IPR004573. rRNA_ssu_MeTfrase_B. IPR023541. rRNA_ssu_MeTfrase_B_ent. [Graphical view]
Pfam	PF01189. Nol1_Nop2_Fmu. 1 hit. PF01029. NusB. 1 hit. [Graphical view]
PRINTS	PR02008. RCMTFAMILY.
SUPFAM	SSF48013. NusB_RsmB_TIM44. 1 hit.
TIGRFAMs	TIGR00563. rsmB. 1 hit.
PROSITE	PS01153. NOL1_NOP2_SUN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSMB_YERPY

Accession

Primary (citable) accession number: B1JJH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	April 29, 2008
Last modified:	May 29, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program