B1JGL9 (NUOH_YERPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit H
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit H
NDH-1 subunit H

Gene names

Name:	nuoH
Ordered Locus Names:	YPK_1566

Organism

Yersinia pseudotuberculosis serotype O:3 (strain YPIII) [Complete proteome] [HAMAP]

Taxonomic identifier

502800 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia › Yersinia pseudotuberculosis

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone By similarity. HAMAP-Rule MF_01350
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01350
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential HAMAP-Rule MF_01350.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	NADH-quinone oxidoreductase subunit H HAMAP-Rule MF_01350		PRO_1000143628
Regions
	Transmembrane	11 – 31	21	Helical; Potential
	Transmembrane	81 – 101	21	Helical; Potential
	Transmembrane	114 – 134	21	Helical; Potential
	Transmembrane	154 – 174	21	Helical; Potential
	Transmembrane	186 – 206	21	Helical; Potential
	Transmembrane	237 – 257	21	Helical; Potential
	Transmembrane	265 – 285	21	Helical; Potential
	Transmembrane	304 – 324	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	B1JGL9 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 53FE4B56D845C0DD	FASTA	325	36,213
	10 20 30 40 50 60 MSWFTPELIE ILISVLKAVV ILLVVVTCGA FMSFGERRLL GLFQNRYGPN RVGWGGSLQL 70 80 90 100 110 120 VADMIKMFFK EDWVPRFSDR AIFTLAPVIA FTSLLLSFAI VPVSPTWAVA DLNIGILFFL 130 140 150 160 170 180 MMAGLAVYAV LFAGWASNNK YSLLGAMRAS AQTLSYEVFL GLSLMGVVAQ VGSFNMQDIV 190 200 210 220 230 240 NSQEHVWNVI PQFFGFLTFA IAGVAVCHRH PFDQPEAEQE LADGYHIEYS GMKFGLFFVG 250 260 270 280 290 300 EYIGIVTVSA LIVTLFFGGW QGPFLPPFIW FALKTAFFMV MFILIRASLP RPRYDQVMSF 310 320 GWKVCLPLTL LNLLATAAVI LYNAQ

References

[1]

"Complete sequence of Yersinia pseudotuberculosis YPIII." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L. , Challacombe J.F., Green L., Lindler L.E., Nikolich M.P., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YPIII.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000950 Genomic DNA. Translation: ACA67859.1.
RefSeq	YP_001720312.1. NC_010465.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	502800.YPK_1566.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA67859; ACA67859; YPK_1566.
GeneID	6091217.
KEGG	ypy:YPK_1566.
PATRIC	18660382. VBIYerPse127545_1680.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1005.
HOGENOM	HOG000228276.
KO	K00337.
OMA	VGEYINI.
ProtClustDB	PRK06076.
Family and domain databases
HAMAP	MF_01350. NDH1_NuoH.
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1/FPO. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
PANTHER	PTHR11432. PTHR11432. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOH_YERPY

Accession

Primary (citable) accession number: B1JGL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program