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B1JF83

- SYI_PSEPW

UniProt

B1JF83 - SYI_PSEPW

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Protein
Isoleucine--tRNA ligase
Gene
ileS, PputW619_4559
Organism
Pseudomonas putida (strain W619)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei567 – 5671Aminoacyl-adenylate By similarity
Binding sitei611 – 6111ATP By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity
Metal bindingi926 – 9261Zinc By similarity
Metal bindingi929 – 9291Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPPUT390235:GHHJ-4628-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PputW619_4559
OrganismiPseudomonas putida (strain W619)
Taxonomic identifieri390235 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000720: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189189Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi390235.PputW619_4559.

Structurei

3D structure databases

ProteinModelPortaliB1JF83.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi608 – 6125"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1JF83-1 [UniParc]FASTAAdd to Basket

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MTDYKATLNL PDTAFPMKAG LPQREPQILQ RWDSIGLYQK LREIGKDRPK    50
FVLHDGPPYA NGKIHIGHAL NKILKDMIVR SKTLSGFDAP YVPGWDCHGL 100
PIEHKVEVTH GKHLTADRTR ELCREYAAEQ IEGQKTEFIR LGVLGDWDNP 150
YKTMNFANEA GEIRALAEMV KQGFVFKGLK PVNWCFDCGS ALAEAEVEYA 200
DKKSQTIDVA FPVADADKLA AAFGLPALAK PAAIVIWTTT PWTIPANQAL 250
NIHPEFKYAL VDTGERLLVL AEELVESCLK RYNLEGSVIA TAQGSALELV 300
NFRHPFYDRL SPVYLADYVE LGAGTGVVHS APAYGEDDFV TCKRYGMVND 350
DILTPVQSNG VYVESLEFFG GQFIWKANPA IVEKLSEVGA LMHTETISHS 400
YMHCWRHKTP LIYRATAQWF VGMDKQPSTG EPLRERALKA IEDTQFVPAW 450
GQARLHSMIA NRPDWCISRQ RNWGVPIPFF LHKQTGELHP RTVELMEAVA 500
KRVEQEGIEA WFKLDAAELL GDEAGQYDKI TDTLDVWFDS GTTHWHVLRG 550
SHDIGHATGP RADLYLEGSD QHRGWFHSSL LTGCAIDNHA PYRELLTHGF 600
TVDESGRKMS KSLGNTIEPE KVNNTLGADI LRLWVSATDY SGEMAVSEQI 650
LQRSADAYRR IRNTARFLLS NLSGFDPARD LLAPEDMLAL DRWAVDRTLL 700
LQRELEEHYS EYRFWNVYSK IHNFCVQELG GFYLDIIKDR QYTTGANSVA 750
RRSCQTALYH ISEALVRWIA PILAFTADEI WQYLPGERNE SVMLNGWYQG 800
LSELPEGTEL DRAYWDRVMA VKAAVNKELE NQRTAKVIGG NLQAEVTLFA 850
EEGLSADLSK LGDELRFVLI TSAASVVPFA QAPADAVATE VEGLKLKVVK 900
SGHAKCGRCW HFRADVGSHP EHPEICSRCV DNLSGSGEVR HYA 943
Length:943
Mass (Da):105,817
Last modified:April 29, 2008 - v1
Checksum:i32EE8EB6B6946E6F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000949 Genomic DNA. Translation: ACA75039.1.
RefSeqiYP_001751408.1. NC_010501.1.

Genome annotation databases

EnsemblBacteriaiACA75039; ACA75039; PputW619_4559.
GeneIDi6113535.
KEGGippw:PputW619_4559.
PATRICi19959029. VBIPsePut93764_4585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000949 Genomic DNA. Translation: ACA75039.1 .
RefSeqi YP_001751408.1. NC_010501.1.

3D structure databases

ProteinModelPortali B1JF83.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 390235.PputW619_4559.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACA75039 ; ACA75039 ; PputW619_4559 .
GeneIDi 6113535.
KEGGi ppw:PputW619_4559.
PATRICi 19959029. VBIPsePut93764_4585.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PPUT390235:GHHJ-4628-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: W619.

Entry informationi

Entry nameiSYI_PSEPW
AccessioniPrimary (citable) accession number: B1JF83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 29, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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