B1JCS1 (ACEK_PSEPW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	PputW619_3866

Organism

Pseudomonas putida (strain W619) [Complete proteome] [HAMAP]

Taxonomic identifier

390235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 569	569	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_1000133274
Regions
	Nucleotide binding	316 – 322	7	ATP By similarity
Sites
	Active site	372	1	By similarity
	Binding site	337	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1JCS1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: EB2535206592D53A	FASTA	569	65,929
	10 20 30 40 50 60 MTQHWPAGEI ARMILDGFDD YREHFRRITL GARERFEQAR WQDIQRAAAA RINLYEQKVG 70 80 90 100 110 120 EVNGWLRDGF DEEVLLEVEQ WPLVKSAYIR LIDPRLDDEL AETWYNSLFC SLFSHDQISD 130 140 150 160 170 180 GCMFIHTTRP SIRSHERAAQ TRTYMPDGSL KGLVRAIFRD YAFDYGDLES DLSRLEEQLR 190 200 210 220 230 240 DCLPDWVCKD PALAVELFVP VLYRNKGAYL VGRLYNSDEQ WPLVIPLLHR EGHGIEADAL 250 260 270 280 290 300 ITDEAEVSII FSFTRSYFMV DVPVPAEFVN FLKRILPGKH IAELYTSIGF YKHGKSEFYR 310 320 330 340 350 360 ALINHLASTD DRFIMAPGVR GMVMSVFTLP GFNTVFKIIK DRFSPSKTVD RATVIDKYRL 370 380 390 400 410 420 VKSVDRVGRM ADTQEFADFR FPRGKFEPEC LAELLEVAPS TVAVEGDTVL VRHCWTERRM 430 440 450 460 470 480 IPLNLYLEQA SEGQVLEALE DYGLAIKQLA AANIFPGDML LKNFGVTRHG RVVFYDYDEI 490 500 510 520 530 540 SYLTEVNFRH IPPPRYPEDE MSGEPWYSIG PHDVFPEEFP PFLFADMGQR RLFSRLHGEL 550 560 YDADYWKGLQ ASIREGKVID VFPYRRKGR

References

[1]

"Complete sequence of Pseudomonas putida W619." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Taghavi S., Vangronsveld D., van der Lelie D., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: W619.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000949 Genomic DNA. Translation: ACA74347.1.
RefSeq	YP_001750716.1. NC_010501.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B1JCS1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6112830.
GenomeReviews	Gene locus PputW619_3866 in contig CP000949_GR.
KEGG	ppw:PputW619_3866.
PATRIC	19957621. VBIPsePut93764_3893.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG298593.
OMA	RMTPLNI.
ProtClustDB	PRK02946.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_PSEPW

Accession

Primary (citable) accession number: B1JCS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program