Reviewed, UniProtKB/Swiss-Prot B1JCH3 (ASTD_PSEPW)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	PputW619_3767

Organism

Pseudomonas putida (strain W619) [Complete proteome] [HAMAP]

Taxonomic identifier

390235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity. HAMAP MF_01174
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_1000138052
Regions
	Nucleotide binding	221 – 226	6	NAD By similarity
Sites
	Active site	244	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1JCH3-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 573A96D48143520F	FASTA	487	51,365
	10 20 30 40 50 60 MTTHYIAGSW QAGQGEALQS LNPVTQAVVW QGQGADASQV DAAVLAARQA FPAWAQLSLE 70 80 90 100 110 120 ARIDVLEKFA EQLKQHSEAL AYCIGEETGK PLWESATEVT SMVNKVAISI QSYRERTGEK 130 140 150 160 170 180 SGPLADATAV LRHKPHGVVA VFGPYNFPGH LPNGHIVPAL LAGNCVVFKP SELTPKVAEL 190 200 210 220 230 240 TVNCWIAAGL PAGVLNLVQG ARETGVALAA NPGIDGLFFT GSSRTGNLLH QQFAGRPDKI 250 260 270 280 290 300 LALEMGGNNP LVVDGVKDLD AAVYTIIQSA FISAGQRCTC ARRLLVPQGA WGDALVIRLV 310 320 330 340 350 360 EVCKSITVGA FDQQPAPFMG SVISLQAARA LVAAQSELLA KGATQLLEMT QPQADAALLT 370 380 390 400 410 420 PGIIDVTAVA DRPDEEFFGP LLQVIRYADF DAAIDEANNT QYGLAAGLLS DSRARYQYFW 430 440 450 460 470 480 LRSRAGIVNW NKQLTGAASS APFGGIGASG NHRASAYYAA DYCAYPVASL ETASLALPAT LTPGVTL

References

[1]

"Complete sequence of Pseudomonas putida W619." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Taghavi S., Vangronsveld D., van der Lelie D., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000949 Genomic DNA. Translation: ACA74249.1.
RefSeq	YP_001750618.1.
3D structure databases
SMR	B1JCH3. Positions 2-462.
ModBase	Search...
Genome annotation databases
GeneID	6112731.
GenomeReviews	Gene locus PputW619_3767 in contig CP000949_GR.
KEGG	ppw:PputW619_3767.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG752218.
OMA	EMTQPQA.
Family and domain databases
HAMAP	MF_01174. Aldedh_AstD. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03240. arg_catab_astD. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_PSEPW

Accession

Primary (citable) accession number: B1JCH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)